Fixatives

Our protocols for perfusions are available either in the histology notebooks in 123D or in the TLab Notebooks google drive Bill's protocol

Berkeley Protocols for working with formalin are (here)

Safety, in brief: Work in the hood or other sufficiently ventilated area (the special perfusion table in NAF necropsy is ok, too). Wear lab coat, closed toed shoes, eye protection, and gloves. Gloves that are contaminated can be washed in the sink and then disposed of in the normal trash. Same with containers. It's fine to keep tissue in fixative in the fridge, but try to label them extremely well- for some reason fixed tissue is always getting left in fridges forever.

Waste pickup:

"Place formaldehyde waste in a labeled, chemically compatible container with a sealed lid. Complete a pickup request at https://jwas.ehs.berkeley.edu/iframe/waste.html#. All biological materials preserved in formaldehyde must also be disposed of in this manner, not in medical waste containers. Formalin solutions may vary in their toxicity, depending on their formulation. Formalin solutions containing less the 2.5% formaldehyde are considered non-toxic. Drain disposal of non-toxic aqueous solutions containing less than 2.5% formaldehyde is permitted to the limit of 100 grams of solute per laboratory per day. This limit applies only as long as no other hazardous chemical is present in the solution. Call EH&S (642-3073) if you have questions about the disposal of formaldehyde waste. See the EH&S Fact Sheet, “Unwanted Hazardous Chemicals,” for general instructions on disposing of hazardous materials."