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bmatern authored May 23, 2017
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#Bhast
# Bhast
Ben's HLA Allele Submission Tool
A tool for generating an EMBL-formatted submission of a standard novel HLA allele.

##Download the executable
## Download the executable
When the compiled executable is available, i'll put a link for the file download here.

##Run using Python
## Run using Python
Run this program using standard python call:
```
python AlleleSubmissionEMBL.py
```

##To configure Anaconda
## To configure Anaconda
Anaconda uses separate environments to run your programs in.
Install Anaconda for python 2.7.
https://www.continuum.io/downloads
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call activate AlleleSubEnvironment && pip install pyinstaller packaging && call deactivate
```

##Run using a bash or .bat script using anaconda
## Run using a bash or .bat script using anaconda
You can execute the following scripts to run the tool inside of Anaconda:
Linux/Mac:
```
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Run_allele_submission.bat
```

##Input Data
## Input Data
Input data sequence must consist of
1) 5' UTR
2) Any number of Exons, with an introns distributed between them.
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AGCTAGCTA
gctagctagctag

##Output Data
## Output Data
The resulting report is in the form of an EMBL HLA Novel Allele submission flatfile. You can submit this to EMBL as a new HLA allele.

##For more information on EMBL's ENA format:
## For more information on EMBL's ENA format:
http://www.ebi.ac.uk/ena/submit/sequence-submission
http://www.ebi.ac.uk/ena/submit/entry-upload-templates
ftp://ftp.ebi.ac.uk/pub/databases/embl/doc/usrman.txt
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