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A tool for generating an EMBL-formatted submission of a standard novel HLA allele.

##Run using Python
Run this program using standard python call:
$python AlleleSubmissionEMBL.py
Run this program using standard python call:
```
python AlleleSubmissionEMBL.py
```

##To configure Anaconda:
Anaconda uses separate environments to run your programs in.
Install Anaconda for python 2.7.
https://www.continuum.io/downloads
To set up the environment in anaconda, run this in console:
$conda create --name AlleleSubEnvironment biopython
Anaconda uses separate environments to run your programs in.
Install Anaconda for python 2.7.
https://www.continuum.io/downloads
To set up the environment in anaconda, run this in console:
```
conda create --name AlleleSubEnvironment biopython
```

##Run using a bash or .bat script using anaconda
You can execute the following scripts to run the tool inside of Anaconda:
Linux/Mac:
bash Run_allele_submission.sh
Windows:
You can execute the following scripts to run the tool inside of Anaconda:
Linux/Mac:
```
bash Run_allele_submission.sh
```
Windows:
```
Run_allele_submission.bat
```

Input data sequence consists of
1) 5' UTR
##Input Data
Input data sequence consists of
1) 5' UTR
2) An odd number of Exons, with an Even number of introns between them.
3) 3' UTR

Introns, Exons, and UTRs are to be specified using capital or lowercase letters.
Exons are capital, while introns and UTRs are lowercase.

Like this:

fiveprimeutrEXONONEintrononeEXONTWOintrontwoEXONTHREEthreeprimeutr

Like this:
fiveprimeutrEXONONEintrononeEXONTWOintrontwoEXONTHREEthreeprimeutr
agctagctagctAGCTAGCtagctagctAGCTAGCtagctagctAGCTAGCTAgctagctagctag

All spaces, tabs, and newlines are not interpreted by this program, They are removed.
If you wish, you may use those characters for more convenient visualization of splice sites:
agctagctagct
AGCTAGC
tagctagct
AGCTAGC
tagctagct
AGCTAGCTA
gctagctagctag

For more information on EMBL's ENA format:

http://www.ebi.ac.uk/ena/submit/sequence-submission

http://www.ebi.ac.uk/ena/submit/entry-upload-templates\n
ftp://ftp.ebi.ac.uk/pub/databases/embl/doc/usrman.txt

ftp://ftp.ebi.ac.uk/pub/databases/embl/doc/FT_current.html

http://www.ebi.ac.uk/ena/software/flat-file-validator
agctagctagct
AGCTAGC
tagctagct
AGCTAGC
tagctagct
AGCTAGCTA
gctagctagctag

##For more information on EMBL's ENA format:
http://www.ebi.ac.uk/ena/submit/sequence-submission
http://www.ebi.ac.uk/ena/submit/entry-upload-templates
ftp://ftp.ebi.ac.uk/pub/databases/embl/doc/usrman.txt
ftp://ftp.ebi.ac.uk/pub/databases/embl/doc/FT_current.html
http://www.ebi.ac.uk/ena/software/flat-file-validator

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