add info for gene TIP2|bHLH142

data/Gene/Abstract/E/EAT1/Connection

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+DTD|EAT1	TIP2|bHLH142	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development.	We revealed that TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1.

data/Gene/Abstract/OS/E/OsERF3/Connection

@@ -4,3 +4,4 @@ AP37|OsERF3	OsACS2	EAR motif mutation of rice OsERF3 alters the regulation of et
 AP37|OsERF3	OsAP2-39	Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice	 Biochemical and molecular approaches showed that OsDERF1 at least directly interacted with the GCC box in the promoters of ERF repressors OsERF3 and OsAP2-39| Moreover, overexpression of OsERF3/OsAP2-39 suppressed ethylene synthesis|Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice
 AP37|OsERF3	OsDERF1	Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice	 Biochemical and molecular approaches showed that OsDERF1 at least directly interacted with the GCC box in the promoters of ERF repressors OsERF3 and OsAP2-39|Transcriptional activation of OsDERF1 in OsERF3 and OsAP2-39 negatively modulates ethylene synthesis and drought tolerance in rice
 AP37|OsERF3	AP59|OsBIERF3	Overexpression of the Transcription Factor AP37 in Rice Improves Grain Yield under Drought Conditions	 Two genes, AP37 and AP59, representing subgroups I and II, respectively, were functionally characterized| The overexpression of AP37 and AP59 in rice under the control of the constitutive promoter OsCc1 increased the tolerance to drought and high salinity at the vegetative stage| Microarray experiments identified 10 and 38 genes that are up-regulated by AP37 and AP59, respectively, in addition to 37 genes that are commonly induced by both factors
+AP37|OsERF3	TIP2|bHLH142	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice	EAT1 positively regulates the expression of AP37 and AP25, which induce tapetal programmed cell death.

data/Gene/Abstract/T/TDR/Connection

@@ -4,3 +4,5 @@ OsC6	TDR	High temperatures cause male sterility in rice plants with transcriptio
 OsC6	TDR	OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice	 Furthermore, additional evidence is provided that the expression of OsC6 is positively regulated by a basic helix-loop-helix transcription factor, Tapetum Degeneration Retardation (TDR)| Extra granule-like structures were observed on the inner surface of the tdr tapetal layer when the expression of OsC6 was driven by the TDR promoter compared with the tdr mutant
 OsC6	TDR	The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development	 More importantly, two genes, Os CP1 and Os c6, encoding a Cys protease and a protease inhibitor, respectively, were shown to be the likely direct targets of TDR through chromatin immunoprecipitation analyses and the electrophoretic mobility shift assay
 OsCP1	TDR	The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development	 More importantly, two genes, Os CP1 and Os c6, encoding a Cys protease and a protease inhibitor, respectively, were shown to be the likely direct targets of TDR through chromatin immunoprecipitation analyses and the electrophoretic mobility shift assay
+TDR	TIP2|bHLH142	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development.	We revealed that TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1.
+TDR	TIP2|bHLH142	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development.	In addition, TIP2 can interact with TDR, indicating a role of TIP2 in later anther development.

data/Gene/Abstract/T/TIP2~bHLH142/Connection

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+TDR	TIP2|bHLH142	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development.	We revealed that TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1.
+DTD|EAT1	TIP2|bHLH142	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development.	We revealed that TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1.
+TDR	TIP2|bHLH142	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development.	In addition, TIP2 can interact with TDR, indicating a role of TIP2 in later anther development.
+TIP2|bHLH142	Udt1	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice	bHLH142 acts downstream of UDT1 and GAMYB but upstream of TDR1 and EAT1 in pollen development.
+AP37|OsERF3	TIP2|bHLH142	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice	EAT1 positively regulates the expression of AP37 and AP25, which induce tapetal programmed cell death.

data/Gene/Abstract/T/TIP2~bHLH142/Keyword.trait

@@ -0,0 +1,7 @@
+Symbol	Keyword	Title	Evidence
+TIP2|bHLH142	transcription factor	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
+TIP2|bHLH142	anther development	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
+TIP2|bHLH142	tapetal programmed cell death	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development	The tip2 mutants display undifferentiated inner three anther wall layers and abort tapetal programmed cell death, causing complete male sterility
+TIP2|bHLH142	male sterility	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development	The tip2 mutants display undifferentiated inner three anther wall layers and abort tapetal programmed cell death, causing complete male sterility
+TIP2|bHLH142	tapetal programmed cell death	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice	Thus, in this study, we identified bHLH142 as having a pivotal role in tapetal programmed cell death and pollen development.
+TIP2|bHLH142	pollen development	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice	Thus, in this study, we identified bHLH142 as having a pivotal role in tapetal programmed cell death and pollen development.

data/Gene/Abstract/T/TIP2~bHLH142/reference.info

@@ -0,0 +1,3 @@
+Publication	Title	Year	Journal	Affiliation	Abstract
+NA	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice	2014	Plant Cell	Academia Sinica Biotechnology Center in Southern Taiwan, Tainan 741, Taiwan Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan	Male sterility plays an important role in F1 hybrid seed production. We identified a male-sterile rice (Oryza sativa) mutant with impaired pollen development and a single T-DNA insertion in the transcription factor gene bHLH142. Knockout mutants of bHLH142 exhibited retarded meiosis and defects in tapetal programmed cell death. RT-PCR and in situ hybridization analyses showed that bHLH142 is specifically expressed in the anther, in the tapetum, and in meiocytes during early meiosis. Three basic helix-loop-helix transcription factors, UDT1 (bHLH164), TDR1 (bHLH5), and EAT1/DTD1 (bHLH141) are known to function in rice pollen development. bHLH142 acts downstream of UDT1 and GAMYB but upstream of TDR1 and EAT1 in pollen development. In vivo and in vitro assays demonstrated that bHLH142 and TDR1 proteins interact. Transient promoter assays demonstrated that regulation of the EAT1 promoter requires bHLH142 and TDR1. Consistent with these results, 3D protein structure modeling predicted that bHLH142 and TDR1 form a heterodimer to bind to the EAT1 promoter. EAT1 positively regulates the expression of AP37 and AP25, which induce tapetal programmed cell death. Thus, in this study, we identified bHLH142 as having a pivotal role in tapetal programmed cell death and pollen development.
+NA	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development	2014	Plant Cell	State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China	In male reproductive development in plants, meristemoid precursor cells possessing transient, stem cell-like features undergo cell divisions and differentiation to produce the anther, the male reproductive organ. The anther contains centrally positioned microsporocytes surrounded by four distinct layers of wall: the epidermis, endothecium, middle layer, and tapetum. Here, we report that the rice (Oryza sativa) basic helix-loop-helix (bHLH) protein TDR INTERACTING PROTEIN2 (TIP2) functions as a crucial switch in the meristemoid transition and differentiation during early anther development. The tip2 mutants display undifferentiated inner three anther wall layers and abort tapetal programmed cell death, causing complete male sterility. TIP2 has two paralogs in rice, TDR and EAT1, which are key regulators of tapetal programmed cell death. We revealed that TIP2 acts upstream of TDR and EAT1 and directly regulates the expression of TDR and EAT1. In addition, TIP2 can interact with TDR, indicating a role of TIP2 in later anther development. Our findings suggest that the bHLH proteins TIP2, TDR, and EAT1 play a central role in regulating differentiation, morphogenesis, and degradation of anther somatic cell layers, highlighting the role of paralogous bHLH proteins in regulating distinct steps of plant cell-type determination.

data/Gene/Abstract/U/Udt1/Connection

@@ -4,3 +4,4 @@ OsMSP1	Udt1	The rice gene DEFECTIVE TAPETUM AND MEIOCYTES 1 (DTM1) is required f
 DTM1	Udt1	The rice gene DEFECTIVE TAPETUM AND MEIOCYTES 1 (DTM1) is required for early tapetum development and meiosis	 Transcripts of UDT1, which functions in tapetum development during early meiosis, were reduced in dtm1 anthers, as were those of PAIR1, which is involved in chromosome pairing and synapsis during meiosis| Moreover, transcripts of DTM1 were reduced in msp1 mutant anthers, but not in udt1 and pair1 mutants| These results, together with their mutant phenotypes, suggest that DTM1 plays important roles in the ER membrane during early tapetum development, functioning after MSP1 and before UDT1, and also in meiocyte development, after MEL1 and before PAIR1
 TDR	Udt1	Identification of gamyb-4 and analysis of the regulatory role of GAMYB in rice anther development	 These results revealed that the expression of TDR (TAPETUM DEGENERATION RETARDATION), a tapetal cell death regulator, was downregulated in gamyb-4 and udt1 (undeveloped tapetum1)| While the GAMYB expression was not obviously changed in tdr and udt1-1, and no apparent expression fold change of UDT1 in tdr and gamyb-4, suggesting that TDR may act downstream of GAMYB and UDT1, and GAMYB and UDT1 work in parallel to regulate rice early anther development
 OsMYBGA|OsGAMYB	Udt1	Identification of gamyb-4 and analysis of the regulatory role of GAMYB in rice anther development	 These results revealed that the expression of TDR (TAPETUM DEGENERATION RETARDATION), a tapetal cell death regulator, was downregulated in gamyb-4 and udt1 (undeveloped tapetum1)| While the GAMYB expression was not obviously changed in tdr and udt1-1, and no apparent expression fold change of UDT1 in tdr and gamyb-4, suggesting that TDR may act downstream of GAMYB and UDT1, and GAMYB and UDT1 work in parallel to regulate rice early anther development
+TIP2|bHLH142	Udt1	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice	bHLH142 acts downstream of UDT1 and GAMYB but upstream of TDR1 and EAT1 in pollen development.

geneKeyword.table

@@ -9981,6 +9981,12 @@ THIS1	Os01g0751600	LOC_Os01g54810	reproductive	THIS1 is a putative lipase that r
 THIS1	Os01g0751600	LOC_Os01g54810	strigolactone	THIS1 is a putative lipase that regulates tillering, plant height, and spikelet fertility in rice
 THIS1	Os01g0751600	LOC_Os01g54810	cell division	THIS1 is a putative lipase that regulates tillering, plant height, and spikelet fertility in rice
 THIS1	Os01g0751600	LOC_Os01g54810	shoot	THIS1 is a putative lipase that regulates tillering, plant height, and spikelet fertility in rice
+TIP2|bHLH142	Os01g0293100	LOC_Os01g18870	transcription factor	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
+TIP2|bHLH142	Os01g0293100	LOC_Os01g18870	anther development	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
+TIP2|bHLH142	Os01g0293100	LOC_Os01g18870	tapetal programmed cell death	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
+TIP2|bHLH142	Os01g0293100	LOC_Os01g18870	male sterility	The Rice Basic Helix-Loop-Helix Transcription Factor TDR INTERACTING PROTEIN2 Is a Central Switch in Early Anther Development
+TIP2|bHLH142	Os01g0293100	LOC_Os01g18870	tapetal programmed cell death	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice
+TIP2|bHLH142	Os01g0293100	LOC_Os01g18870	pollen development	The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice
 TOM1|OsZIFL4	Os11g0134900	LOC_Os11g04020	transporter	Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants
 TOM1|OsZIFL4	Os11g0134900	LOC_Os11g04020	iron	Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants
 TOM1|OsZIFL4	Os11g0134900	LOC_Os11g04020	root	Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants