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Update scrna_seq.md (theislab#203)
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Correct MinIO -> MinION.
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ddiez authored Jun 1, 2023
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Expand Up @@ -86,7 +86,7 @@ First, long-read sequencing, which describes the ability to obtain nucleotide fr

Pacific Biosciences' (PacBio) introduced zero-mode waveguide (ZMW) sequencing in 2010, which uses so-called nanoholes containing a single DNA polymerase. This allows incorporation of any single nucleotide to be directly observed by detectors attached below the nanoholes. Each type of nucleotides is labeled with a specific fluorescent dye that emits fluorescent signals during the incorporation process, which are subsequently measured as sequence readout. Reads obtained from PacBio sequencers are usually of 8 to 15 kilobases (kb), with possibilities for up to 70kb.

Oxford Nanopore Technologies' introduced the GridION in 2012. The GridION and its successors MinIO and Flongle are portable sequencers for DNA and RNA sequencing which produce reads of more than 2 Mb. Notably, such a sequencing device even fits into a single human hand. Oxford Nanopore sequencers observe changes in the electrical current that occur when nucleic acids pass through protein nanopores, to identify the nucleotide sequence{cite}`Jain2016`.
Oxford Nanopore Technologies' introduced the GridION in 2012. The GridION and its successors MinION and Flongle are portable sequencers for DNA and RNA sequencing which produce reads of more than 2 Mb. Notably, such a sequencing device even fits into a single human hand. Oxford Nanopore sequencers observe changes in the electrical current that occur when nucleic acids pass through protein nanopores, to identify the nucleotide sequence{cite}`Jain2016`.

Strengths:

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