version 0.0.1
First release
pyrpipe: python rna-seq pipeliner
Introduction
pyrpipe (Pronounced as "pyre-pipe") is a python package to easily develop RNA-Seq analyses workflow by integrating popular RNA-Seq analysis programs in an object oriented manner.
pyrpipe can be used on local computers or on HPC environments to manage analysis of RNA-Seq data. Users can use the easy-to-use APIs to popular bioinformatic tools provided
with pyrpipe, or can use the methods provided in the pyrpipe_engine module to integrate any other third-party program in their pipeline.
What it does
Allows fast and easy development of bioinformatics pipelines in python by providing
- a high level api to popular bioinformatics tools
- a general api to execute any linux command from python (using the subprocess module)
- comprehensive logging features to log all the commands, output and their return status
- report generating features for easy sharing, reproducing, benchmarking and debugging
Prerequisites
- python 3.6 or higher
- OS: Linux, Mac
pyrpipe provides API to:
Tool | Purpose |
---|---|
SRA Tools (v. 2.9.6 or higher) | SRA access |
Trimgalore | QC |
BBDuk | QC |
Hisat2 | Alignment |
STAR | Alignment |
Bowtie2 | Alignment |
Kallisto | Quant |
Salmon | Quant |
Stringtie | Transcript Assembly |
Cufflinks | Transcript Assembly |
Trinity | Transcript Assembly |
Samtools | Tools |
Portcullis | Tools |
Mikado | Tools |