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Update README.md
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sujaikumar committed Apr 28, 2014
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Expand Up @@ -456,8 +456,9 @@ Requirements:
2. Local copy of NCBI blast database (eg: nt or nr)
3. Install BLAST+ suite from ftp://ftp.ncbi.nih.gov/blast/executables/blast+/LATEST

`curl "http://www.ncbi.nlm.nih.gov/sviewer/viewer.fcgi?tool=portal&sendto=on&db=nuccore&dopt=gilist&qty=2000000&filter=all&term=txid1224\[Organism:exp\]" >txid1224.gids`
`blastdb_aliastool -dbtype nucl -gilist txid1224.gids -db /path/to/blastdb/nt -out nt_Proteobacteria`
curl "http://www.ncbi.nlm.nih.gov/sviewer/viewer.fcgi?tool=portal&sendto=on&db=nuccore&dopt=gilist&qty=2000000&filter=all&term=txid1224\[Organism:exp\]" >txid1224.gids
blastdb_aliastool -dbtype nucl -gilist txid1224.gids -db /path/to/blastdb/nt -out nt_Proteobacteria

Remember:

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