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This repository contains workflow for moving window analysis (MWA). This is carried out on secondary metabolite producing BGC genes of bacterial origin.

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somakchowdhury/mwa-secmet-bgc

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mwa-secmet-bgc

This repository contains workflow for moving-window-analysis (MWA) carried out on secondary metabolite BGC genes of bacterial origin

Documentation --> https://mwa-secmet-bgc.readthedocs.io/en/latest/index.html

The workflow requires the following preinstalled components on the system.

  • A linux sub-system
  • Initialised miniconda package manager
  • Jupyter installed in the base environment of miniconda.

Step 1:

  • Navigate to your directory
 cd path/to/your/dir 
  • Clone this repository in that directory
git clone https://github.com/somakchowdhury/mwa-secmet-bgc.git 

Step 2:

  • Navigate to mwa-secmet-bgc folder
  • Then run this command
jupyter notebook
  • In the browser open the jupyter notebook file .ipynb

Follow through the steps listed in the workflow.

Expected Outputs

Four unnumbered output folders will be created at the end of the workflow.

mwa-secmet-bgc
|___fragmented_pairs/
|___blast_out/
|___result_plots/
|___result_tables/

The results_plot/ contains pairwise graphs of the compared sequences with appropriate titles for subject and query

Example plot

The result_tables/ contains modified and filtered blast output tables that were used to produce the plots.

Example table

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This repository contains workflow for moving window analysis (MWA). This is carried out on secondary metabolite producing BGC genes of bacterial origin.

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