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Merge pull request #1294 from atrigila/smrnaseq
Add smrnaseq trimmed fastq test data
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sample,fastq_1 | ||
Clone1_N1,https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/trimmed/small_Clone1_N1.fastp.fastq.gz | ||
Clone1_N3,https://github.com/nf-core/test-datasets/raw/smrnaseq/testdata/trimmed/small_Clone1_N3.fastp.fastq.gz |
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The data in this directory contains input files to start the pipeline from different protocols or settings. | ||
1. Trimmed reads obtained from fastp. Useful to test the pipeline with the flag `--skip_fastp`: `trimmed` directory |
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Sample data from FASTP. This data was obtained running `nextflow run smrnaseq -profile test,docker -r dev --save_merged` with the following custom `publishDir`: | ||
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``` | ||
withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTP' { | ||
ext.args = [ "", | ||
params.trim_fastq ? "" : "--disable_adapter_trimming", | ||
params.clip_r1 > 0 ? "--trim_front1 ${params.clip_r1}" : "", // Remove bp from the 5' end of read 1. | ||
params.three_prime_clip_r1 > 0 ? "--trim_tail1 ${params.three_prime_clip_r1}" : "", // Remove bp from the 3' end of read 1 AFTER adapter/quality trimming has been performed. | ||
params.fastp_min_length > 0 ? "-l ${params.fastp_min_length}" : "", | ||
params.fastp_max_length > 0 ? "--max_len1 ${params.fastp_max_length}" : "", | ||
params.three_prime_adapter == "auto-detect" ? "" : "--adapter_sequence ${params.three_prime_adapter}" | ||
].join(" ").trim() | ||
publishDir = [ | ||
[ | ||
path: { "${params.outdir}/fastp/fastq" }, | ||
mode: params.publish_dir_mode, | ||
pattern: "*.fastp.fastq.gz", | ||
enabled: params.save_merged | ||
] | ||
] | ||
} | ||
``` | ||
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Size was reduced with: `zcat Clone1_N3.fastp.fastq.gz | head -n 4000000 | gzip > small_Clone1_N3.fastp.fastq.gz` |
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