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Update docs/usage.md
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Co-authored-by: Nicolás Schcolnicov <[email protected]>
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atrigila and nschcolnicov authored Nov 12, 2024
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Expand Up @@ -47,7 +47,7 @@ If MirGeneDB should be used instead it needs to be specified using `--mirgenedb`

See examples in: [the test-datasets repository of nf-core](https://github.com/nf-core/test-datasets/tree/smrnaseq/MirGeneDB).

> [!NOTE] > `mirtop` is hard-coded to use the `pre` sequences rather than `pri`. Users must provide `pre` files from the start to ensure consistency between the FASTA and GFF files, as the coordinates in the GFF file are referenced to `pre` sequences. This also ensures that names in the BAM file will match those in the GFF.
> [!NOTE] > `mirtop` is hard-coded to use the `pre` sequences, which originate from the hairpin FASTA, rather than the `pri` sequences, which come from the mature FASTA. Users must provide `pre` files from the start to ensure consistency between the FASTA and GFF files, as the coordinates in the GFF file are referenced to `pre` sequences. This also ensures that names in the BAM file will match those in the GFF.
### Genome

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