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Msatfinder-2.0.9, taken from: | ||
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http://web.archive.org/web/20071026090642/http://www.genomics.ceh.ac.uk/msatfinder/ |
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Msatfinder README, Last Updated July 2006 | ||
Authors: Milo Thurston ([email protected]) and Dawn Field | ||
([email protected]) | ||
The latest version of this software is available at: | ||
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http://www.bioinf.ceh.ac.uk/msatfinder/downloads/msatfinder-latest.tar.gz | ||
or | ||
http://www.bioinf.ceh.ac.uk/msatfinder/downloads/msatfinder-latest.zip | ||
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This file describes the installation and usage of the msatfinder program | ||
for the detection of microsatellite repeats. For full instructions, | ||
please see the msatfinder user manual. Please send comments, suggestions, | ||
or bug reports to the authors. | ||
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INSTALLATION | ||
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1. Download the latest version of the msatfinder tar/zip file and unpack | ||
it. | ||
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[~user] tar zxvf msatfinder-latest.tar.gz * | ||
[~user] cd msatfinder-<version> | ||
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*or unzip msatfinder-latest.zip | ||
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2. If you require that the program be available to all users, then | ||
copy the script to your preferred location, e.g. | ||
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[msatfinder-<version>] sudo cp msatfinder /usr/local/bin | ||
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3. Make sure the following dependencies are installed and configured on | ||
your system, or on your PATH in the case of EMBOSS &c.: | ||
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Perl modules: | ||
- Bioperl | ||
- CGI | ||
- Config::Simple | ||
- File::Copy | ||
- File::Basename | ||
- Getopt::Std | ||
- Term::ReadLine | ||
- List::Util | ||
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Software: | ||
EMBOSS (eprimer3) | ||
Primer3_core | ||
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BASIC CONFIGURATION AND USAGE | ||
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To check that your package and all dependencies are installed and | ||
configured correctly, test data is supplied (two baculovirus genomes in | ||
Genbank format). | ||
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1. Read through the default values in msatfinder.rc, and change anything that | ||
you think might require altering (e.g. types and thresholds of msats to be | ||
searched for). This is probably not necessary for most searches. | ||
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2. To run the search, type: | ||
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./msatfinder *gbk | ||
or | ||
./msatfinder *sprot | ||
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An example Swissprot and Genbank file is provided. | ||
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Output from this search will be found in the directories Fasta/, | ||
Repeats/, Msat_tabs/, Flank_tabs/, and Primers/. An html index | ||
containing hyperlinks to all the output files will be created as | ||
Repeats/results.html. Open in your preferred web browser to view | ||
the results. | ||
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For further details on how to set up searches of your own data, please see the | ||
Msatfinder user manual. | ||
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If you experience any problems running this software, please write to | ||
the authors. |
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