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olivierrue committed Jun 7, 2024
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Visit our web site : http://frogs.toulouse.inrae.fr/

[![Release](https://img.shields.io/badge/release-4.1.0-blue.svg)![Date](https://img.shields.io/badge/date-March%202023-red.svg)](https://github.com/geraldinepascal/FROGS-wrappers/releases) [<img src="https://cdn.icon-icons.com/icons2/70/PNG/512/deezer_14086.png" width="2%" style="display: block; margin: auto;"/>](https://www.deezer.com/fr/playlist/5233843102?utm_source=deezer&utm_content=playlist-5233843102&utm_term=18632989_1545296531&utm_medium=web)
[![Release](https://img.shields.io/badge/release-5.0.0-blue.svg)![Date](https://img.shields.io/badge/date-June%202024-red.svg)](https://github.com/geraldinepascal/FROGS-wrappers/releases) [<img src="https://cdn.icon-icons.com/icons2/70/PNG/512/deezer_14086.png" width="2%" style="display: block; margin: auto;"/>](https://www.deezer.com/fr/playlist/5233843102?utm_source=deezer&utm_content=playlist-5233843102&utm_term=18632989_1545296531&utm_medium=web)



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| Denoising and Remove_chimera | [vsearch](https://github.com/torognes/vsearch) | 2.17.0 |
| Denoising | [flash](https://sourceforge.net/projects/flashpage/files/) (optional) | 1.2.11 |
| Denoising | [cutadapt](https://github.com/marcelm/cutadapt) (need to be >=2.8) | 2.10 |
| Denoising | [swarm](https://github.com/torognes/swarm) (need to be >=2.1) | 3.0.0 |
| Denoising | [swarm](https://github.com/torognes/swarm) (need to be >=2.1) | 3.1.4 |
| Denoising | [DADA2](https://benjjneb.github.io/dada2/index.html) | 1.22.0 |
| ITSx | [ITSx](http://microbiology.se/software/itsx/) | 1.1.2 |
| Taxonomic_affiliation | [NCBI BLAST+](http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastDocs&DOC_TYPE=Download) | 2.10 |
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If you work in an academic lab on a private Galaxy server, or if you have paid your license you can use PEAR in FROGS preprocess.
For that you need to:

- have PEAR in your PATH or in the FROGS libexec directory. We have tested PEAR 0.9.10 version (last version 0.9.11).
- have PEAR in your PATH or in the FROGS libexec directory. We have tested PEAR 0.9.11 version.
- use `--merge-software pear` option in the preprocess.py command line

## FROGS and dependencies installation
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Step frogsfunc_functions jeu. 02 mai 2024 18:33:49 CEST
Step frogsfunc_pathways jeu. 02 mai 2024 18:36:32 CEST
Completed with success
Completed with success
```


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