Dependencies:
- python 3.6.5
- Biopython 1.7
- matplotlib 2.1.1
- seaborn 0.8.1
- pandas 0.22.0
- numpy 1.14.0
- cutadapt 1.16
See ./gRNA_selection for instructions on selecting gRNAs for use with Sp-Cas9/Sp-dCas9
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scripts for pooled genome-wide CRISPRi screen in V. natriegens
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