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[add virus config] config + resources for h3n2 ha segement - thx anika
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LaraFuhrmann committed May 8, 2024
1 parent e669520 commit 011524d
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75 changes: 75 additions & 0 deletions config/h3n2_ha.yaml
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# config-file used for the analysis of H3N2 segment HA
# config file was used to analysis data from SRA available
# through the SRA Run accession: SRP385331

general:
aligner: bwa
primers_trimmer: samtools
threads: 6
snv_caller: lofreq
temp_prefix: ./temp
preprocessor: skip

input:
datadir: samples/
samples_file: samples.tsv
reference: "{VPIPE_BASEDIR}/../resources/h3n2_ha/h3n2_ha.fasta"
genes_gff: "{VPIPE_BASEDIR}/../resources/h3n2_ha/gffs/h3n2_ha.gff3"
paired: true
read_length: 151

output:
datadir: results/
snv: True
local: True
global: False
visualization: False
QA: False
diversity: False

gunzip:
mem: 100000

extract:
mem: 100000

preprocessing:
mem: 10000

sam2bam:
mem: 5000

ref_bwa_index:
mem: 65536

bwa_align:
mem: 40690
threads: 8

bowtie_align:
mem: 12288
threads: 6

coverage:
mem: 131072
threads: 32
time: 60

minor_variants:
mem: 16384
threads: 64

coverage_intervals:
coverage: 0
mem: 2000
threads: 1

lofreq:
consensus: false

snv:
consensus: false
localscratch: $TMPDIR
time: 240
mem: 1024
threads: 64
5 changes: 5 additions & 0 deletions resources/h3n2_ha/gffs/h3n2_ha.gff3
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##gff-version 3
##sequence-region EPI1857216 1 1718
EPI1857216 feature gene 1 48 . + . gene_name="SigPep"
EPI1857216 feature gene 49 1035 . + . gene_name="HA1"
EPI1857216 feature gene 1036 1698 . + . gene_name="HA2"
23 changes: 23 additions & 0 deletions resources/h3n2_ha/h3n2_ha.fasta
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>EPI_ISL_1563628 | A/Darwin/6/2021 | A / H3N2 | | 2021-03-16
ATGAAGACTATCATTGCTTTGAGCAACATTCTATGTCTTGTTTTCGCTCAAAAAATACCTGGAAATGACAATAGCACGGC
AACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAACAATCACAAATGACCGAATTGAAGTTACTA
ATGCTACTGAGTTGGTTCAGAATTCATCAATAGGTGAAATATGCGGCAGTCCTCATCAGATCCTTGATGGAGGGAACTGC
ACACTAATAGATGCTCTATTGGGGGACCCTCAGTGTGACGGCTTTCAAAATAAGGAATGGGACCTTTTTGTTGAAAGAAG
CAGAGCCAACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACAC
TGGAGTTTAAAAATGAAAGCTTCAATTGGACTGGAGTCAAACAAAACGGAACAAGTTCTGCGTGCATAAGGGGATCTAGT
AGTAGTTTTTTTAGTAGATTAAATTGGTTGACCAGCTTAAACAACATATATCCAGCACAGAACGTGACTATGCCAAACAA
GGAACAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGATACGGACAAGAACCAAATCTCCCTGTTTGCTCAAT
CATCAGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATCGGATCTAGACCCAGAATAAGG
GATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCT
AATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGTA
AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCGTTCCAAAATGTAAACAGGATCACATACGGGGCC
TGTCCCAGATATGTTAAGCAAAGCACCCTGAAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAAACCAGAGGCAT
ATTTGGCGCAATAGCGGGTTTCATAGAAAATGGATGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATT
CTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTG
ATCGGAAAAACCAACGAGAAATTCCATCAGATTGAAAAAGAATTCTCAGAAGTAGAAGGAAGAGTTCAAGACCTTGAGAA
ATATGTTGAGGACACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAGAACCAACATACGATTG
ACCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGAAAT
GGTTGTTTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATAAGAAATGAAACTTATGACCACAATGTGTA
CAGGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCTATGGA
TTTCCTTTGCCATGTCATGTTTTTTGCTTTGTATTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATT
AGATGCAACATTTGCATTTGAGTGCATTAATTAAAAAC

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