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[add virus config] config + resources for h3n2 ha segement - thx anika
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# config-file used for the analysis of H3N2 segment HA | ||
# config file was used to analysis data from SRA available | ||
# through the SRA Run accession: SRP385331 | ||
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general: | ||
aligner: bwa | ||
primers_trimmer: samtools | ||
threads: 6 | ||
snv_caller: lofreq | ||
temp_prefix: ./temp | ||
preprocessor: skip | ||
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input: | ||
datadir: samples/ | ||
samples_file: samples.tsv | ||
reference: "{VPIPE_BASEDIR}/../resources/h3n2_ha/h3n2_ha.fasta" | ||
genes_gff: "{VPIPE_BASEDIR}/../resources/h3n2_ha/gffs/h3n2_ha.gff3" | ||
paired: true | ||
read_length: 151 | ||
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output: | ||
datadir: results/ | ||
snv: True | ||
local: True | ||
global: False | ||
visualization: False | ||
QA: False | ||
diversity: False | ||
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gunzip: | ||
mem: 100000 | ||
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extract: | ||
mem: 100000 | ||
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preprocessing: | ||
mem: 10000 | ||
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sam2bam: | ||
mem: 5000 | ||
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ref_bwa_index: | ||
mem: 65536 | ||
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bwa_align: | ||
mem: 40690 | ||
threads: 8 | ||
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bowtie_align: | ||
mem: 12288 | ||
threads: 6 | ||
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coverage: | ||
mem: 131072 | ||
threads: 32 | ||
time: 60 | ||
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minor_variants: | ||
mem: 16384 | ||
threads: 64 | ||
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coverage_intervals: | ||
coverage: 0 | ||
mem: 2000 | ||
threads: 1 | ||
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lofreq: | ||
consensus: false | ||
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snv: | ||
consensus: false | ||
localscratch: $TMPDIR | ||
time: 240 | ||
mem: 1024 | ||
threads: 64 |
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##gff-version 3 | ||
##sequence-region EPI1857216 1 1718 | ||
EPI1857216 feature gene 1 48 . + . gene_name="SigPep" | ||
EPI1857216 feature gene 49 1035 . + . gene_name="HA1" | ||
EPI1857216 feature gene 1036 1698 . + . gene_name="HA2" |
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>EPI_ISL_1563628 | A/Darwin/6/2021 | A / H3N2 | | 2021-03-16 | ||
ATGAAGACTATCATTGCTTTGAGCAACATTCTATGTCTTGTTTTCGCTCAAAAAATACCTGGAAATGACAATAGCACGGC | ||
AACGCTGTGCCTTGGGCACCATGCAGTACCAAACGGAACGATAGTGAAAACAATCACAAATGACCGAATTGAAGTTACTA | ||
ATGCTACTGAGTTGGTTCAGAATTCATCAATAGGTGAAATATGCGGCAGTCCTCATCAGATCCTTGATGGAGGGAACTGC | ||
ACACTAATAGATGCTCTATTGGGGGACCCTCAGTGTGACGGCTTTCAAAATAAGGAATGGGACCTTTTTGTTGAAAGAAG | ||
CAGAGCCAACAGCAACTGTTACCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCATCCGGCACAC | ||
TGGAGTTTAAAAATGAAAGCTTCAATTGGACTGGAGTCAAACAAAACGGAACAAGTTCTGCGTGCATAAGGGGATCTAGT | ||
AGTAGTTTTTTTAGTAGATTAAATTGGTTGACCAGCTTAAACAACATATATCCAGCACAGAACGTGACTATGCCAAACAA | ||
GGAACAATTTGACAAATTGTACATTTGGGGGGTTCACCACCCGGATACGGACAAGAACCAAATCTCCCTGTTTGCTCAAT | ||
CATCAGGAAGAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATCGGATCTAGACCCAGAATAAGG | ||
GATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCT | ||
AATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAGATGCACCCATTGGCAAATGTA | ||
AGTCTGAATGCATCACTCCAAATGGAAGCATTCCCAATGACAAACCGTTCCAAAATGTAAACAGGATCACATACGGGGCC | ||
TGTCCCAGATATGTTAAGCAAAGCACCCTGAAATTGGCAACAGGAATGCGAAATGTACCAGAGAAACAAACCAGAGGCAT | ||
ATTTGGCGCAATAGCGGGTTTCATAGAAAATGGATGGGAGGGAATGGTGGATGGTTGGTACGGTTTCAGGCATCAAAATT | ||
CTGAGGGAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGCTGAATCGATTG | ||
ATCGGAAAAACCAACGAGAAATTCCATCAGATTGAAAAAGAATTCTCAGAAGTAGAAGGAAGAGTTCAAGACCTTGAGAA | ||
ATATGTTGAGGACACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAGAACCAACATACGATTG | ||
ACCTAACTGACTCAGAAATGAACAAACTGTTTGAAAAAACAAAGAAGCAACTGAGGGAAAATGCTGAGGATATGGGAAAT | ||
GGTTGTTTCAAAATATACCACAAATGTGACAATGCCTGCATAGGATCAATAAGAAATGAAACTTATGACCACAATGTGTA | ||
CAGGGATGAAGCATTAAACAACCGGTTCCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGGATCCTATGGA | ||
TTTCCTTTGCCATGTCATGTTTTTTGCTTTGTATTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCAAAAGGGCAACATT | ||
AGATGCAACATTTGCATTTGAGTGCATTAATTAAAAAC |