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MAINT: update README #39

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42 changes: 41 additions & 1 deletion README.md
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# q2-moshpit
![CI](https://github.com/bokulich-lab/q2-moshpit/actions/workflows/ci.yaml/badge.svg)
![QIIME CI](https://github.com/bokulich-lab/q2-moshpit/actions/workflows/q2-ci.yaml/badge.svg)
[![codecov](https://codecov.io/gh/bokulich-lab/q2-moshpit/graph/badge.svg?token=PSCAYJUP01)](https://codecov.io/gh/bokulich-lab/q2-moshpit)

**MO**dular **SH**otgun metagenome **P**ipelines with **I**ntegrated provenance **T**racking
QIIME 2 plugin for functional annotation and taxonomic classification of shotgun metagenomes.

## Installation
_q2-moshpit_ is available as part of the QIIME 2 metagenome distribution. For installation and usage instructions please consult the official [QIIME 2 documentation](https://www.docs.qiime2.org).

## Functionality
This QIIME 2 plugin contains actions used to annotate and classify (meta)genomes.
Below you will find an overview of actions available in the plugin.

| Action | Description | Underlying tool |
|----------------------|------------------------------------------------------------|--------------------------------------------------------------------|
| bin-contigs-metabat | Bin contigs into MAGs using MetaBat 2. | [MetaBat 2](https://bitbucket.org/berkeleylab/metabat/src/master/) |
| build-custom-diamond-db | Create a DIAMOND reference database from a FASTA input file. | [Diamond](https://github.com/bbuchfink/diamond) |
| build-eggnog-diamond-db | Create a DIAMOND reference database for the specified taxon. | [Diamond](https://github.com/bbuchfink/diamond) |
| build-kraken-db | Fetch an existing or build a custom Kraken 2 database. | [Kraken 2](https://ccb.jhu.edu/software/kraken2/) |
| classify-kaiju | Classify reads using Kaiju. | [Kaiju](https://bioinformatics-centre.github.io/kaiju/) |
| classify-kraken2 | Classify reads/MAGs using Kraken 2. | [Kraken 2](https://ccb.jhu.edu/software/kraken2/) |
| dereplicate-mags | Dereplicate MAGs from multiple samples. | - |
| eggnog-annotate | Annotate orthologs against eggNOG database. | [EggNOG mapper](https://github.com/eggnogdb/eggnog-mapper) |
| eggnog-diamond-search | Run eggNOG search using diamond aligner. | [EggNOG mapper](https://github.com/eggnogdb/eggnog-mapper) |
| eggnog-hmmer-search | Run eggNOG search using HMMER aligner. | [EggNOG mapper](https://github.com/eggnogdb/eggnog-mapper) |
| estimate-bracken | Perform read abundance re-estimation using Bracken. | [Kraken 2](https://ccb.jhu.edu/software/bracken/) |
| estimate-mag-abundance | Estimate MAG abundance. | - |
| evaluate-busco | Evaluate quality of the generated MAGs using BUSCO. | [BUSCO](https://busco.ezlab.org) |
| extract-annotations | Extract annotation frequencies from all annotations. | - |
| fetch-busco-db | Download BUSCO database. | [BUSCO](https://busco.ezlab.org) |
| fetch-diamond-db | Fetch the complete Diamond database necessary to run the eggnog-diamond-search action. | [EggNOG mapper](https://github.com/eggnogdb/eggnog-mapper) |
| fetch-eggnog-db | Fetch the databases necessary to run the eggnog-annotate action. | [EggNOG mapper](https://github.com/eggnogdb/eggnog-mapper) |
| fetch-eggnog-hmmer-db | Fetch the taxon specific database necessary to run the eggnog-hmmer-search action. | [EggNOG mapper](https://github.com/eggnogdb/eggnog-mapper) |
| fetch-eggnog-proteins | Fetch the databases necessary to run the build-eggnog-diamond-db action. | [EggNOG mapper](https://github.com/eggnogdb/eggnog-mapper) |
| fetch-kaiju-db | Fetch Kaiju database. | [Kaiju](https://bioinformatics-centre.github.io/kaiju/) |
| fetch-ncbi-taxonomy | Fetch NCBI reference taxonomy. | [EggNOG mapper](https://github.com/eggnogdb/eggnog-mapper) |
| filter-derep-mags | Filter dereplicated MAGs. | - |
| filter-mags | Filter MAGs. | - |
| filter-reads-pangenome | Remove contaminating human reads. | [Bowtie 2](https://bowtie-bio.sourceforge.net/bowtie2/index.shtml) |
| get-feature-lengths | Get feature lengths. | - |
| inspect-kraken2-db | Inspect a Kraken 2 database. |
| kraken2-to-features | Select downstream features from Kraken 2. | - |
| kraken2-to-mag-features | Select downstream MAG features from Kraken 2. | - |
| multiply-tables | Multiply two feature tables. | - |
| predict-genes-prodigal | Predict gene sequences from MAGs using Prodigal. | [Prodigal](https://github.com/hyattpd/Prodigal) |
10 changes: 10 additions & 0 deletions environment-files/q2-moshpit-qiime2-metagenome-2024.10.yml
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channels:
- https://packages.qiime2.org/qiime2/2024.10/metagenome/passed
- conda-forge
- bioconda
dependencies:
- qiime2-metagenome
- pip
- pip:
- q2-moshpit@git+https://github.com/bokulich-lab/q2-moshpit.git@main

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