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ENH: Adds new transformer for AMRFinderPlusAnnotationsDirFmt to qiime2.Metadata #6

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Oct 11, 2024
Merged

ENH: Adds new transformer for AMRFinderPlusAnnotationsDirFmt to qiime2.Metadata #6

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46a2879
added new transformer for annotations to metadata with tests
VinzentRisch Sep 26, 2024
fa87025
added transfomers import to plugin setup
VinzentRisch Sep 26, 2024
1026eec
added files to package data
VinzentRisch Sep 26, 2024
4f76e2e
merge 3
VinzentRisch Sep 26, 2024
2aab907
merge main
VinzentRisch Oct 9, 2024
f3865cf
removed create append df
VinzentRisch Oct 9, 2024
c6ccc0b
merge 7
VinzentRisch Oct 11, 2024
4bf3977
typo
VinzentRisch Oct 11, 2024
7463e4a
modified transformer
VinzentRisch Oct 11, 2024
f14afa2
combine_index_test modification
VinzentRisch Oct 11, 2024
4ff2bb6
Merge branch 'main' into 5_transformer_metadata
VinzentRisch Oct 11, 2024
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4 changes: 4 additions & 0 deletions q2_amrfinderplus/plugin_setup.py
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#
# The full license is in the file LICENSE, distributed with this software.
# ----------------------------------------------------------------------------
import importlib

from q2_types.feature_data import FeatureData
from q2_types.feature_data_mag import MAG
from q2_types.genome_data import Genes, GenomeData, Loci, Proteins
Expand Down Expand Up @@ -270,3 +272,5 @@
AMRFinderPlusAnnotationFormat,
AMRFinderPlusAnnotationsDirFmt,
)

importlib.import_module("q2_amrfinderplus.types._transformer")
55 changes: 55 additions & 0 deletions q2_amrfinderplus/types/_transformer.py
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# ----------------------------------------------------------------------------
# Copyright (c) 2019-2023, QIIME 2 development team.
#
# Distributed under the terms of the Modified BSD License.
#
# The full license is in the file LICENSE, distributed with this software.
# ----------------------------------------------------------------------------
import pandas as pd
import qiime2

from q2_amrfinderplus.plugin_setup import plugin
from q2_amrfinderplus.types import AMRFinderPlusAnnotationsDirFmt


@plugin.register_transformer
def _1(data: AMRFinderPlusAnnotationsDirFmt) -> qiime2.Metadata:
return qiime2.Metadata(_metadata_transformer_helper(data))


def _metadata_transformer_helper(data):
df_list = []

if any(item.is_dir() for item in data.path.iterdir()):
annotation_dict = data.annotation_dict()

else:
file_dict = data.annotation_dict()
# Create annotation_dict with fake sample
annotation_dict = {"": file_dict}

for outer_id, files_dict in annotation_dict.items():
for inner_id, file_fp in files_dict.items():
id_value = f"{outer_id}/{inner_id}" if outer_id else inner_id

# Create df and append to df_list
df = pd.read_csv(file_fp, sep="\t")
df.insert(0, "Sample/MAG_ID", id_value)
df_list.append(df)

return combine_dataframes(df_list)


def combine_dataframes(df_list):
# Concat all dfs
df_combined = pd.concat(df_list, axis=0)

# Sort all values by sample/mag ID column
df_combined.sort_values(by=df_combined.columns[0], inplace=True)

# Reset and rename index and set it to string to conform to metadata format
df_combined.reset_index(inplace=True, drop=True)
df_combined.index.name = "id"
df_combined.index = df_combined.index.astype(str)

return df_combined
3 changes: 3 additions & 0 deletions ...ta/annotations_feature_data_mags/aa447c99-ecd9-4c4a-a53b-4df6999815dd_amr_annotations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...ta/annotations_feature_data_mags/e026af61-d911-4de3-a957-7e8bf837f30d_amr_annotations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...mrfinderplus/types/tests/data/annotations_sample_data_contigs/sample1_amr_annotations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...mrfinderplus/types/tests/data/annotations_sample_data_contigs/sample2_amr_annotations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...tations_sample_data_mags/sample1/e026af61-d911-4de3-a957-7e8bf837f30d_amr_annotations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...tations_sample_data_mags/sample2/aa447c99-ecd9-4c4a-a53b-4df6999815dd_amr_annotations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
5 changes: 5 additions & 0 deletions q2_amrfinderplus/types/tests/data/metadata_tables/feature_data.tsv
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Sample/MAG_ID Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aa447c99-ecd9-4c4a-a53b-4df6999815dd aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.0 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
aa447c99-ecd9-4c4a-a53b-4df6999815dd blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.0 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
e026af61-d911-4de3-a957-7e8bf837f30d aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.0 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
e026af61-d911-4de3-a957-7e8bf837f30d blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.0 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
5 changes: 5 additions & 0 deletions q2_amrfinderplus/types/tests/data/metadata_tables/sample_data_contigs.tsv
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Sample/MAG_ID Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
sample1 aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.0 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
sample1 blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.0 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
sample2 aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.0 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
sample2 blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.0 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
5 changes: 5 additions & 0 deletions q2_amrfinderplus/types/tests/data/metadata_tables/sample_data_mags.tsv
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Sample/MAG_ID Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
sample1/e026af61-d911-4de3-a957-7e8bf837f30d aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.0 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
sample1/e026af61-d911-4de3-a957-7e8bf837f30d blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.0 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
sample2/aa447c99-ecd9-4c4a-a53b-4df6999815dd aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.0 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
sample2/aa447c99-ecd9-4c4a-a53b-4df6999815dd blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.0 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...ta/mutations_feature_data_mags/aa447c99-ecd9-4c4a-a53b-4df6999815dd_amr_all_mutations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...ta/mutations_feature_data_mags/e026af61-d911-4de3-a957-7e8bf837f30d_amr_all_mutations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...mrfinderplus/types/tests/data/mutations_sample_data_contigs/sample1_amr_all_mutations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...mrfinderplus/types/tests/data/mutations_sample_data_contigs/sample2_amr_all_mutations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...tions_sample_data_mags/sample1/e026af61-d911-4de3-a957-7e8bf837f30d_amr_all_mutations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
3 changes: 3 additions & 0 deletions ...tions_sample_data_mags/sample2/aa447c99-ecd9-4c4a-a53b-4df6999815dd_amr_all_mutations.tsv
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Protein identifier Gene symbol Sequence name Scope Element type Element subtype Class Subclass Method Target length Reference sequence length % Coverage of reference sequence % Identity to reference sequence Alignment length Accession of closest sequence Name of closest sequence HMM id HMM description Hierarchy node
aph3pp-Ib_partial_5p_neg aph(3'')-Ib aminoglycoside O-phosphotransferase APH(3'')-Ib core AMR AMR AMINOGLYCOSIDE STREPTOMYCIN PARTIALP 225 267 81.27 100.00 217 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib NF032896.1 APH(3'') family aminoglycoside O-phosphotransferase aph(3'')-Ib
blaOXA-436_partial blaOXA OXA-48 family class D beta-lactamase core AMR AMR BETA-LACTAM BETA-LACTAM PARTIALP 233 265 87.92 100.00 233 WP_058842180.1 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-436 NF012161.0 class D beta-lactamase blaOXA-48_fam
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