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[codespell] | ||
skip = .git,env,venv,ds*,asl*,qmr*,genetics*,fnirs*,eeg*,meg*,pet*,ieeg*,motion*,synthetic* | ||
builtin = clear,rare | ||
ignore-words-list = nd | ||
ignore-words-list = nd,te |
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1 human brain sample imaged over 1 session. | ||
Example dataset with empty images containing X-ray phase-contrast tomography (XPCT) data to illustrate BIDS convention. | ||
Using NGFF nested directory format example |
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from pathlib import Path | ||
import zarr.convenience | ||
import dask.array | ||
import ome_zarr, ome_zarr.io, ome_zarr.writer | ||
from numcodecs import Blosc, Delta | ||
import time | ||
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""" | ||
You may use the following command to prepare a Python 3.8+ environment for the download of the dataset: `pip install -r data_retrieval_reqs.txt` | ||
""" | ||
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data_path = Path("../sub-LADAF-2020-31/ses-01/micr/") # path to session | ||
data_path.mkdir(exist_ok=True) # create directory | ||
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# short utility function | ||
def zarr_array(url,selector): | ||
print('Retrieving data from', url) | ||
n5_store = zarr.N5FSStore(url) | ||
root = zarr.group(store=n5_store) | ||
return dask.array.from_zarr(root[selector]) | ||
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dataset_full = zarr_array("gs://ucl-hip-ct-35a68e99feaae8932b1d44da0358940b/LADAF-2020-31/brain/25.08um_complete-organ_bm05/","s0") # get a dask.array.Array that points to the whole N5 dataset | ||
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# default filters and compressors made the script crash; the ones below work: | ||
filters = [Delta(dtype='i4')] | ||
compressor = Blosc(cname='zstd', clevel=1, shuffle=Blosc.SHUFFLE) | ||
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path_roi = data_path / "sub-LADAF-2020-31_ses-01_sample-brain_XPCT.ome.zarr" # full name of the dataset following BIDS specification | ||
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print('Writing in', str(path_roi)) | ||
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tic = time.time() | ||
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store = ome_zarr.io.parse_url(path_roi,mode="a").store # NB: `mode="a"` should allow overwrite but it does not at the moment, see https://github.com/ome/ome-zarr-py/issues/376 | ||
root = zarr.group(store=store) | ||
ome_zarr.writer.write_image(image=dataset_full, | ||
group=root, | ||
scaler=None, | ||
axes=[ | ||
{ | ||
"name": "z", | ||
"type": "space", | ||
"units": "micrometer" # voxel size is an isotropic 25.08 um | ||
}, | ||
{ | ||
"name": "y", | ||
"type": "space", | ||
"units": "micrometer" # voxel size is an isotropic 25.08 um | ||
}, | ||
{ | ||
"name": "x", | ||
"type": "space", | ||
"units": "micrometer" # voxel size is an isotropic 25.08 um | ||
} | ||
], # axis order of the dataset following BIDS specification | ||
coordinate_transformations=[ | ||
[ | ||
{ | ||
"scale": [ | ||
25.08, | ||
25.08, | ||
25.08 | ||
], # voxel size is an isotropic 25.08 um | ||
"type": "scale" | ||
} | ||
] | ||
], | ||
storage_options=dict( | ||
chunks=(512,512,512), # this chunk size may be altered depending on someone's needs | ||
filters=filters, # default filters made the script crash | ||
compressor=compressor # default compressors made the script crash | ||
) | ||
) | ||
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toc = time.time() | ||
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print('Writing completed in', (toc - tic)/60, 'min!') |
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dask[complete] | ||
zarr | ||
ome-zarr | ||
numcodecs | ||
gcsfs |
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import urllib.request | ||
import json | ||
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""" | ||
You may use the following command to prepare a Python 3.8+ environment for the download of the dataset: `pip install -r metadata_formatter_reqs.txt` | ||
""" | ||
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# The following metadata file can be downloaded from https://human-organ-atlas.esrf.eu/datasets/572252538 | ||
metadata_txt_file_uri = 'https://ids.esrf.fr/ids/getData?sessionId=182d0a3b-de3b-4602-8caf-9bd91dc5b0e5&datafileIds=572252539' # This URI expires quite often | ||
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req = urllib.request.urlopen(metadata_txt_file_uri) | ||
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json_dict = {} | ||
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for line in urllib.request.urlopen(metadata_txt_file_uri): | ||
text = line.decode('utf-8') | ||
if text.startswith('#') or text == '\r\n': | ||
pass | ||
else: | ||
s = text.replace('\t','').replace('\r\n','').replace('"', '').replace('N.A.', 'n/a').split('=') | ||
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try: | ||
s[1] = int(s[1]) | ||
except: | ||
try: | ||
s[1] = float(s[1]) | ||
except: | ||
pass | ||
json_dict[s[0]] = s[1] | ||
json_obj = json.dumps(json_dict) | ||
print(json_obj) | ||
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""" | ||
The metadata has been split into several files: | ||
- micr_XPCTzarr/samples.json | ||
- micr_XPCTzarr/sub-LADAF-2020-31/sub-LADAF-2020-31_sessions.tsv | ||
- micr_XPCTzarr/sub-LADAF-2020-31/ses-01/micr/sub-LADAF-2020-31_ses-01_sample-brain_XPCT.json | ||
Note that some fields are redundant. | ||
""" |
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urllib3 |
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#!/bin/bash | ||
# Bash script to truncate all the files containing the binary chunked data as per ../../CONTRIBUTING.md | ||
# Modified from ../../CONTRIBUTING.md | ||
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find ../sub-LADAF-2020-31/ses-01/micr/sub-LADAF-2020-31_ses-01_sample-brain_XPCT.ome.zarr/ -type f -regex ".*/[0-9]*" -exec truncate -s 0 {} + |
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{ | ||
"Name": "micr_XPCTzarr", | ||
"Authors": [ | ||
"Matthieu Chourrout", | ||
"David Stansby", | ||
"Guillaume Gaisne", | ||
"Claire L. Walsh", | ||
"Peter D. Lee" | ||
], | ||
"BIDSVersion": "1.10.0", | ||
"License": "CC-BY-4.0", | ||
"DatasetDOI": "doi:10.15151/ESRF-DC-572252655", | ||
"DatasetType": "raw", | ||
"HowToAcknowledge": "Please cite this dataset as: Tafforeau, P., Walsh, C., Wagner, W. L., Daniyal J. Jafree, Bellier, A., Werlein, C., Kühnel, M. P., Boller, E., Walker-Samuel, S., Robertus, J. L., Long, D. A., Jacob, J., Marussi, S., Eeline Brown, Holroyd, N., Jonigk, D. D., Ackermann, M., & Lee, P. D. (2021). Complete brain from the body donor LADAF-2020-31 (Version 1) [dataset]. European Synchrotron Radiation Facility. https://doi.org/10.15151/ESRF-DC-572252655" | ||
} |
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{ | ||
"participant_id": { | ||
"Description": "Unique alphanumeric participant ID starting with sub-" | ||
}, | ||
"participant_name": { | ||
"Description": "full name or pseudo of the participant which can contain non-alphanumeric characters" | ||
}, | ||
"sex": { | ||
"Description": "sex of the participant", | ||
"Levels": { | ||
"M": "male", | ||
"F": "female" | ||
} | ||
}, | ||
"age": { | ||
"Description": "age of the participant", | ||
"Units": "year" | ||
}, | ||
"weight": { | ||
"Description": "weight of the participant", | ||
"Units": "kg" | ||
}, | ||
"height": { | ||
"Description": "height of the participant", | ||
"Units": "cm" | ||
}, | ||
"species": { | ||
"Description": "binomial species name from the NCBI Taxonomy (https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi)" | ||
}, | ||
"medical_information": { | ||
"Description": "medical information as provided by the biobank" | ||
}, | ||
"institute": { | ||
"Description": "name of the biobank" | ||
} | ||
} |
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participant_id participant_name sex age species weight height institute medical_information | ||
sub-01 LADAF-2020-31 F 69 homo sapiens 40 145 Laboratoire d'Anatomie des Alpes Françaises type 2 diabetes, pelvic radiation to treat cancer of the uterus, right colectomy (benign lesion on histopathology), bilateral nephrostomy for acute obstructive renal failure, cystectomy, omentectomy and peritoneal carcinoma with occlusive syndrome |
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{ | ||
"sample_id": { | ||
"Description": "Sample ID" | ||
}, | ||
"participant_id": { | ||
"Description": "Participant ID from whom tissue samples have been acquired" | ||
}, | ||
"sample_type": { | ||
"Description": "Type of sample from ENCODE Biosample Type (https://www.encodeproject.org/profiles/biosample_type)" | ||
}, | ||
"sample_info": { | ||
"Description": "One-line title of the sample" | ||
}, | ||
"sample_preparation": { | ||
"Description": "Specific preparation of the sample" | ||
} | ||
} |
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sample_id participant_id sample_type sample_info sample_preparation | ||
sample-brain sub-01 tissue complete brain from the body donor program of the Laboratoire d'Anatomie des Alpes Francaise (LADAF) formalin fixed, progressive transfer to ethanol 70% with gentle vacuum degassing at each step, mounted with mixed agar gel at 70% ethanol, n.b. some damages due to the too rapid vacuum degassing |
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micr_XPCTzarr/sub-01/ses-01/micr/sub-01_ses-01_sample-brain_XPCT.json
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{ | ||
"InstitutionName": "European Synchrotron Radiation Facility", | ||
"StationName": "BM05 EBS dipole wiggler 0.85T", | ||
"BodyPart": "BRAIN", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "formalin", | ||
"SampleEmbedding": "mixed agar gel at 70% ethanol", | ||
"PixelSize": [ | ||
25.08, | ||
25.08, | ||
25.08 | ||
], | ||
"PixelSizeUnits": "um", | ||
"AcquisitionParameters": { | ||
"XStep": "n/a", | ||
"XStages": 1, | ||
"YStep": "n/a", | ||
"YStages": 1, | ||
"ZStep ": "2.2 mm", | ||
"ZStages ": "2x79", | ||
"Projections": 9990, | ||
"RefN": "n/a", | ||
"DarkN": 400, | ||
"RefOn": "n/a", | ||
"ScanningMode ": "continuous", | ||
"ExposureTime": "0.036 s", | ||
"AccExposureTime ": "0.006 s", | ||
"AccFramesCount": 6, | ||
"PropDistance": "3475 mm", | ||
"Filters": "Mo 0.1mm Al 2mm SiO2 bars 12*5mm diameter", | ||
"DetAvgEnergy": "93 keV", | ||
"ScanGeometry": "quarter-acquisition, one scan in half-acquisition plus one annular scan", | ||
"ScanRange": "360 deg", | ||
"SensorName": "sCMOS PCO edge 4.2 CLHS", | ||
"SensorMode": "rolling shutter", | ||
"SensorPixelSize": "6.5 um", | ||
"Magnification": 0.24, | ||
"XPixelNum": 2048, | ||
"YPixelNum": 176, | ||
"OpticsType": "dzoom optic from BM05 based on Hasselblad 120mm F/4 macro objective", | ||
"Scintillator": "LuAG:Ce 2000 um", | ||
"SurfDoseRate": "10.5 Gy/s", | ||
"VoiDoseRate": "10.5 Gy/s", | ||
"VoiIntegDose": "2.48 kGy", | ||
"ScanTime": "7.88 min", | ||
"SeriesTime": "22 h" | ||
}, | ||
"ProcessingParameters": { | ||
"RefApproach": "reference jar with 70% ethanol, single reference", | ||
"VolumeX": 5965, | ||
"VolumeY": 5965, | ||
"VolumeZ": 6990, | ||
"32to16BitsMin": -0.04, | ||
"32to16BitsMax": 0.1, | ||
"Jp2ComprRatio": 10 | ||
} | ||
} |
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micr_XPCTzarr/sub-01/ses-01/micr/sub-01_ses-01_sample-brain_XPCT.ome.zarr/.zattrs
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{ | ||
"multiscales": [ | ||
{ | ||
"axes": [ | ||
{ | ||
"name": "z", | ||
"type": "space", | ||
"units": "micrometer" | ||
}, | ||
{ | ||
"name": "y", | ||
"type": "space", | ||
"units": "micrometer" | ||
}, | ||
{ | ||
"name": "x", | ||
"type": "space", | ||
"units": "micrometer" | ||
} | ||
], | ||
"datasets": [ | ||
{ | ||
"coordinateTransformations": [ | ||
{ | ||
"scale": [ | ||
25.08, | ||
25.08, | ||
25.08 | ||
], | ||
"type": "scale" | ||
} | ||
], | ||
"path": "0" | ||
} | ||
], | ||
"name": "/", | ||
"version": "0.4" | ||
} | ||
] | ||
} |
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