Skip to content

ENCODE pipeline (under active development in CPU folder)

Pre-release
Pre-release
Compare
Choose a tag to compare
@theaidenlab theaidenlab released this 08 Nov 21:16
· 288 commits to encode since this release

Differences from Juicer 1.5.6:

  • bwa mem –SP5M paired end mode; most recent BWA release 0.7.17
  • nofrag default
  • no short end read alignment anymore
  • alignonly, mergeonly, deduponly stages added
  • simplification of chimeric_blacklist in terms of output stem
  • chimeric_blacklist producing partition of reads into bams:
    - alignable.bam with duplicates marked
    - collisions.bam
    (those two ideally ENCODE product that one can download)
    - collisions_low_mapq.bam
    - unmapped.bam
    - mapq0.bam
  • mitochondria no longer treated as a special case
  • collisions now include contigs as well (blacklist instead of whitelist)
    The merged_nodups file still appears as part of the pipeline. To recover merged_nodups from alignable.bam produced by ENCODE (for example), use the bamtotxt.sh script