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Load Your Own Data into a Sorting | ||
================================= | ||
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Why make a :code:`Sorting`? | ||
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SpikeInterface contains pre-build readers for the output of many common sorters. | ||
However, what if you have sorting output that is not in a standard format (e.g. | ||
old csv file)? If this is the case you can make your own Sorting object to load | ||
your data into SpikeInterface. This means you can still easily apply various | ||
downstream analyses to your results (e.g. building correlograms or for generating | ||
a :code:`SortingAnalyzer``). | ||
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The Sorting object is a core object within SpikeInterface that acts as a convenient | ||
way to interface with sorting results, no matter which sorter was used to generate | ||
them. **At a fundamental level it is a series of spike times and a series of labels | ||
for each unit and a sampling frequency for transforming frames to time.** Below, we will show you have | ||
to take your existing data and load it as a SpikeInterface :code:`Sorting` object. | ||
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Reading a standard spike sorting format into a :code:`Sorting` | ||
------------------------------------------------------------- | ||
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For most spike sorting output formats the :code:`Sorting` is automatically generated. For example one could do | ||
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.. code-block:: python | ||
from spikeinterface.extractors import read_phy | ||
# For kilosort/phy output files we can use the read_phy | ||
# most formats will have a read_xx that can used. | ||
phy_sorting = read_phy('path/to/folder') | ||
And voilà you now have your :code:`Sorting` object generated and can use it for further analysis. For all the | ||
current formats see :ref:`compatible_formats`. | ||
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Loading your own data into a :code:`Sorting` | ||
------------------------------------------- | ||
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This :code:`Sorting` contains important information about your spike trains including: | ||
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* spike times: the peaks of the extracellular potentials expressed in samples/frames these can | ||
be converted to seconds under the hood using the sampling_frequency | ||
* spike labels: the neuron id for each spike, can also be called cluster ids or unit ids | ||
Stored as the :code:`unit_ids` in SpikeInterface | ||
* sampling_frequency: the rate at which the recording equipment was run at. Note this is the | ||
frequency and not the period. This value allows for switching between samples/frames to seconds | ||
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There are 3 options for loading your own data into a sorting object | ||
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With lists of spike trains and spike labels | ||
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ | ||
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In this case we need a list of spike times unit labels, sampling_frequency and optional unit_ids | ||
if you want specific labels to be used (in this case we only create the :code:`Sorting` based on | ||
the requested unit_ids). | ||
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.. code-block:: python | ||
import numpy as np | ||
from spikeinterface.core import NumpySorting | ||
# in this case we are making a monosegment sorting | ||
# we have four spikes that are spread among two neurons | ||
my_sorting = NumpySorting.from_times_labels( | ||
times_list=[ | ||
np.array([1000,12000,15000,22000]) # Note these are samples/frames not times in seconds | ||
], | ||
labels_list=[ | ||
np.array(["a","b","a","b"]) | ||
], | ||
sampling_frequency=30_000.0 | ||
) | ||
With a unit dictionary | ||
^^^^^^^^^^^^^^^^^^^^^^ | ||
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We can also use a dictionary where each unit is a key and its spike times are values. | ||
This is entered as either a list of dicts with each dict being a segment or as a single | ||
dict for monosegment. We still need to separately specify the sampling_frequency | ||
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.. code-block:: python | ||
from spikeinterface.core import NumpySorting | ||
my_sorting = NumpySorting.from_unit_dict( | ||
units_dict_list={ | ||
'0': [1000,15000], | ||
'1': [12000,22000], | ||
}, | ||
sampling_frequency=30_000.0 | ||
) | ||
With Neo SpikeTrains | ||
^^^^^^^^^^^^^^^^^^^^ | ||
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Finally since SpikeInterface is tightly integrated with the Neo project you can create | ||
a sorting from :code:`Neo.SpikeTrain` objects. See :doc:`Neo documentation<neo:index>` for more information on | ||
using :code:`Neo.SpikeTrain`'s. | ||
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.. code-block:: python | ||
from spikeinterface.core import NumpySorting | ||
# neo_spiketrain is a Neo spiketrain object | ||
my_sorting = NumpySorting.from_neo_spiketrain_list( | ||
neo_spiketrain, | ||
sampling_frequency=30_000.0, | ||
) | ||
Loading multisegment data into a :code:`Sorting` | ||
----------------------------------------------- | ||
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One of the great advantages of SpikeInterface :code:`Sorting` objects is that they can also handle | ||
multisegment recordings and sortings (e.g. you have a baseline, stimulus, post-stimulus). The | ||
exact same machinery can be used to generate your sorting, but in this case we do a list of arrays instead of | ||
a single list. Let's go through one example for using :code:`from_times_labels`: | ||
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.. code-block:: python | ||
import numpy as np | ||
from spikeinterface.core import NumpySorting | ||
# in this case we are making three-segment sorting | ||
# we have four spikes that are spread among two neurons | ||
# in each segment | ||
my_sorting = NumpySorting.from_times_labels( | ||
times_list=[ | ||
np.array([1000,12000,15000,22000]), | ||
np.array([30000,33000, 41000, 47000]), | ||
np.array([50000,53000,64000,70000]), | ||
], | ||
labels_list=[ | ||
np.array([0,1,0,1]), | ||
np.array([0,0,1,1]), | ||
np.array([1,0,1,0]), | ||
], | ||
sampling_frequency=30_000.0 | ||
) | ||
Next steps | ||
---------- | ||
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Now that we've created a Sorting object you can combine it with a Recording to make a | ||
:ref:`SortingAnalyzer<sphx_glr_tutorials_core_plot_4_sorting_analyzer.py>` | ||
or start visualizing using plotting functions from our widgets model such as | ||
:py:func:`~spikeinterface.widgets.plot_crosscorrelograms`. |
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.. _release0.100.5: | ||
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SpikeInterface 0.100.5 release notes | ||
------------------------------------ | ||
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6th April 2024 | ||
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Minor release with bug fixes | ||
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* Open Ephys: Use discovered recording ids to load sync timestamps (#2655) | ||
* Fix channel gains in NwbRecordingExtractor with backend (#2661) | ||
* Fix depth location in spikes on traces map (#2676) |
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