This repository is for the Spring 2022 BST281 final project
Link to the proposal: https://docs.google.com/document/d/15N5rx_pjfoQD_6f8W_LaUt3aAc_6FFi62MjR01RT-eI/edit?usp=sharing
Link to the final write-up: https://docs.google.com/document/d/1qfbJqAXosRNAHvteRfodbaY0pdiPJ4LDSLeRzaHcmso/edit#
Please see the following steps for our single-nucleus RNA-seq analysis. For each step below, we create a corresponding folder. Please go into each folder and check out the description.
Step1: Data process, UMAP, and cluster annotation.
Step2: Differentially expressed genes (DEGs) identification
Step3: GO and KEGG pathway enrichement analysis
Step4: Cell-cell communication analysis (CellphoneDB)