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dportik committed Sep 28, 2020
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4 changes: 2 additions & 2 deletions README.md
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Expand Up @@ -8,7 +8,7 @@ Welcome! Here you can find a variety of tools and pipelines tailored to using Pa

+ **`Taxonomic-Functional-Profiling-Protein`**: Align HiFi reads to a **protein** database using DIAMOND and prepare inputs for MEGAN6, for the purpose of taxonomic and functional profiling. Provides access to NCBI and GTDB taxonomic analysis, and allows functional analyses based on annotations from several databases (SEED, InterPro2GO, eggNOG).

+ **`Taxonomic-Functional-Profiling-Nucelotide`**: Align HiFi reads to a **nucleotide** database using minimap2 and prepare inputs for MEGAN6, for the purpose of taxonomic profiling. Only provides access to NCBI taxonomic analysis, but may give higher resolution than with protein alignments. The GTDB taxonomy and functional annotations are not accessible downstream.
+ **`Taxonomic-Profiling-Nucleotide`**: Align HiFi reads to a **nucleotide** database using minimap2 and prepare inputs for MEGAN6, for the purpose of taxonomic profiling. This only provides access to NCBI taxonomic analysis, but may give higher resolution than with protein alignments. The GTDB taxonomy and functional annotations are not accessible downstream.

Each of these pipelines can be found in their respective folders. They are made available as [Snakemake](https://snakemake.readthedocs.io/en/stable/index.html) workflows. Snakemake is a python-based workflow manager. Snakemake workflows are highly portable because dependencies and environments are automatically setup using [Anaconda](https://docs.anaconda.com/anaconda/)/[Conda](https://docs.conda.io/projects/conda/en/latest/index.html). Snakemake also allows reproducibility, checkpointing, and the ability to scale workflows using HPC and cloud environments.

Expand All @@ -19,7 +19,7 @@ All available documentation can be found in the `docs/` folder above.
Currently available documentation:
- [Tutorial: Genome-Binning-Pipeline](https://github.com/PacificBiosciences/pb-metagenomics-tools/blob/master/docs/Tutorial-Genome-Binning-Pipeline.md)
- [Tutorial: Taxonomic-Functional-Profiling-Protein](https://github.com/PacificBiosciences/pb-metagenomics-tools/blob/master/docs/Tutorial-Taxonomic-Functional-Profiling-Protein.md)
- [Tutorial: Taxonomic-Functional-Profiling-Nucleotide](https://github.com/PacificBiosciences/pb-metagenomics-tools/blob/master/docs/Tutorial-Taxonomic-Functional-Profiling-Nucleotide.md)
- [Tutorial: Taxonomic-Profiling-Nucleotide](https://github.com/PacificBiosciences/pb-metagenomics-tools/blob/master/docs/Tutorial-Taxonomic-Profiling-Nucleotide.md)



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2 changes: 1 addition & 1 deletion docs/Tutorial-Genome-Binning-Pipeline.md
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# Genome-Binning-Pipeline <a name="TOP"></a>
# Tutorial for Genome-Binning-Pipeline <a name="TOP"></a>

## **Table of Contents**

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4 changes: 2 additions & 2 deletions docs/Tutorial-Taxonomic-Functional-Profiling-Protein.md
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# Taxonomic-Functional-Profiling-Protein <a name="TOP"></a>
# Tutorial for Taxonomic-Functional-Profiling-Protein <a name="TOP"></a>

## **Table of Contents**

Expand Down Expand Up @@ -235,7 +235,7 @@ For information on how to run snakemake with AWS (Amazon Web Services), Google C
Successful runs will result in several new directories:

```
Genome-Binning-Pipeline
Taxonomic-Functional-Profiling-Protein
├── configs/
├── envs/
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