Merge pull request #16 from jmattick/update-docs-for-v2.9.0

Update docs for v2.9.0
PacificBiosciences · Dec 12, 2023 · a3c3178 · a3c3178
2 parents c8ab581 + 05259f1
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diff --git a/docs/changelog.md b/docs/changelog.md
@@ -6,7 +6,13 @@ nav_order: 99
 
 # Version changelog
 
- * **2.6.0**:
+ * **2.9.0**:
+   * SMRT Link v13.0 release
+   * Add `--overwrite-biosample-name` option for double demux
+   * Add `sa`, `sm`, and `sx` tags
+   * Fixes for `--single-end` 
+
+ * 2.6.0:
    * Support SEGMENT read type
    * Use final MM/ML basemod tags
    * Bug fixes

diff --git a/docs/faq/isoseq.md b/docs/faq/isoseq.md
@@ -28,3 +28,9 @@ Those options are very conservative to remove any spurious and ambiguous
 calls, in order to guarantee that only proper asymmetric (barcoded) primer
 are used in downstream analyses. Good libraries reach >75% CCS reads passing
 *lima* filters.
+
+## Demultiplexing cDNA barcoded adapters after SMRTbell adapter-level demultiplexing
+[Iso-Seq](https://isoseq.how/clustering/cli-workflow.html#step-2---primer-removal-and-demultiplexing) 
+supports pooled cDNA barcoded analysis. If using barcoded cDNA primers after adapter-level demultiplexing, 
+add `--overwrite-biosample-names` to replace the [bio sample names](https://lima.how/faq/biosample.html) assigned during the first round of 
+demultiplexing.