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Update isoseq version 4.1.0 (#54)
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* Update isoseq version 4.1.0

* Fix formatting

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Co-authored-by: Jessica Mattick <[email protected]>
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jmattick and Jessica Mattick authored Nov 7, 2023
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6 changes: 5 additions & 1 deletion docs/changelog.md
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---

# Version changelog
* **4.0.0**
* **4.1.0**
* Update minimap2 to version 2.26
* SMRT Link v13.0 release

* 4.0.0
* Rename `isoseq3` to `isoseq`
* Add new tool `isoseq cluster2`
* Update `--max-5p-diff` default value for `isoseq collapse`
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5 changes: 4 additions & 1 deletion docs/classification/pigeon-input.md
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## Pigeon Tools Input

**prepare**

| File | Description |
| ----------- | ---- |
| Transcript GFF | Collapsed transcript GFF from _isoseq collapse_ |
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| Intropolis data (Optional) | Intropolis data in custom format |

**classify**

| File | Description |
| ----------- | ---- |
| Reference Genome | Reference genome in FASTA format |
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| FL Counts (Optional) | Bulk IsoSeq FL count info from _isoseq collapse_ (`*.flnc_counts.txt`) |

**make-seurat**

| File | Description |
| ----------- | ---- |
| Molecules FASTA | Molecules FASTA file from _isoseq dedup_ |
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### Examples
Sorted and indexed references for human and mouse can be found [here](https://downloads.pacbcloud.com/public/dataset/MAS-Seq/REF-pigeon_ref_sets/).

Guidance about custom references can be found [here] (/classification/pigeon-annotation).
Guidance about custom references can be found [here](/classification/pigeon-annotation).
2 changes: 2 additions & 0 deletions docs/clustering/cli-workflow.md
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Expand Up @@ -36,10 +36,12 @@ sequences in this [lima Iso-Seq FAQ](https://lima.how/faq/isoseq).
$ lima movieX.hifi_reads.bam barcoded_primers.fasta movieX.fl.bam --isoseq --peek-guess

If your sample is barcoded at the cDNA level, use `--overwrite-biosample-names` to update the sample names.

$ lima movieX.hifi_reads.bam barcoded_primers.fasta movieX.fl.bam --isoseq --peek-guess --overwrite-biosample-names

**Example 1:**
Following is the IsoSeq_v2_primers_12.fasta for the MAS-Seq bulk Iso-Seq primers that supports 12-plex barcoding at the cDNA level:

>IsoSeqX_bc01_5p
CTACACGACGCTCTTCCGATCTACTACACGCAATGAAGTCGCAGGGTTGGG
>IsoSeqX_bc02_5p
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