Skip to content

Commit

Permalink
added warning comment about estimating depth by filesize
Browse files Browse the repository at this point in the history
  • Loading branch information
williamrowell committed Sep 28, 2023
1 parent 4273515 commit 2dc2760
Showing 1 changed file with 1 addition and 1 deletion.
2 changes: 1 addition & 1 deletion workflows/de_novo_assembly_trio/de_novo_assembly_trio.wdl
Original file line number Diff line number Diff line change
Expand Up @@ -52,7 +52,7 @@ workflow de_novo_assembly_trio {
# if parental coverage is low (<15x), keep singleton kmers from parents and use them to bin child reads
# if parental coverage is high (>=15x), use bloom filter and require that a kmer occur >= 5 times in
# one parent and <2 times in the other parent to be used for binning
# 60GB uncompressed FASTA ~= 10x coverage
# 60GB uncompressed FASTA ~= 10x coverage (this is not robust to big changes in mean read length)
# memory for 24 threads is 48GB with bloom filter (<=50x coverage) and 65GB without bloom filter (<=30x coverage)
Boolean low_depth = if ((size(samtools_fasta_father.reads_fasta, "GB") < 90) && (size(samtools_fasta_mother.reads_fasta, "GB") < 90)) then true else false
Expand Down

0 comments on commit 2dc2760

Please sign in to comment.