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Mahsa-Ehsanifard committed Jul 21, 2024
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7 changes: 7 additions & 0 deletions README.html
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Expand Up @@ -465,6 +465,13 @@ <h2>DEG analysis</h2>
function in <em>logarithm</em> scale</p></li>
</ul>
<p><code>{r} fit &lt;- lmFit(Ex, design = design) contrast &lt;- makeContrasts(non_V600E-v600e, levels = design) fit2 &lt;- contrasts.fit(fit = fit, contrasts = contrast) fit2 &lt;- eBayes(fit2) deg &lt;- topTable(fit2, number = Inf)</code></p>
<pre><code> logFC AveExpr t P.Value adj.P.Val B
ENSG00000114302 2.059983 9.016187 9.425437 5.230565e-18 7.353333e-14 30.26257
ENSG00000135709 -1.866375 2.860313 -9.361123 8.118053e-18 7.353333e-14 29.83572
ENSG00000106733 -1.558516 2.832475 -9.152146 3.356666e-17 1.843978e-13 28.45755
ENSG00000157954 -2.057048 2.989810 -9.123564 4.071489e-17 1.843978e-13 28.27013
ENSG00000164944 1.905614 7.507759 9.077754 5.544899e-17 2.009028e-13 27.97029
ENSG00000187098 -1.353065 2.716279 -9.004784 9.056078e-17 2.533801e-13 27.49412</code></pre>
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10 changes: 9 additions & 1 deletion README.md
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Expand Up @@ -133,10 +133,18 @@ fit2 <- eBayes(fit2)
deg <- topTable(fit2, number = Inf)
```

```
logFC AveExpr t P.Value adj.P.Val B
ENSG00000114302 2.059983 9.016187 9.425437 5.230565e-18 7.353333e-14 30.26257
ENSG00000135709 -1.866375 2.860313 -9.361123 8.118053e-18 7.353333e-14 29.83572
ENSG00000106733 -1.558516 2.832475 -9.152146 3.356666e-17 1.843978e-13 28.45755
ENSG00000157954 -2.057048 2.989810 -9.123564 4.071489e-17 1.843978e-13 28.27013
ENSG00000164944 1.905614 7.507759 9.077754 5.544899e-17 2.009028e-13 27.97029
ENSG00000187098 -1.353065 2.716279 -9.004784 9.056078e-17 2.533801e-13 27.49412
```



![](https://github.com/Mahsa-Ehsanifard/GEO_microarray/blob/main/voom.png?raw=true)



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