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update README.md with embplant_mt notes
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Kinggerm committed Feb 20, 2021
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Expand Up @@ -117,8 +117,8 @@ or in a slow and memory-economic way:
To assembly Embryophyta plant mitochondria (usually you need more than 5G raw data):

get_organelle_from_reads.py -1 forward.fq -2 reverse.fq -o mitochondria_output -R 50 -k 21,45,65,85,105 -P 1000000 -F embplant_mt
# 1. embplant_mt mode was not tested in the GetOrganelle paper
# 2. until further updates, the FASTA output of plant mitochondria genome of numerous repeats may be error-prone, please use the FASTG file for downstream manual processing.
# 1. please use the FASTG file as the final output for downstream manual processing. until further updates, the FASTA output of plant mitochondria genome of numerous repeats may be error-prone
# 2. embplant_mt mode was not tested in the GetOrganelle paper due to the complexity of plant mitogenomes and the defects of short reads

To assembly Embryophyta plant nuclear ribosomal RNA (18S-ITS1-5.8S-ITS2-26S):

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