CELL-MET-iPSC-cardiomyocytes-Automation

OT-2 API V2 protocol scripts for Opentrons robot with material for using OT-2 liquid handler to perform iPSC-derived cardiomyocytes culturing and differentiation.

This workflow enables iPSC differentiation into cardiomyocytes via Wnt signaling pathway using the OT2 liquid handling robot by Opentrons. This includes:

• Accutase splitting and seeding of induced pluripotent stem cells (iPSCs)
• Induced pluripotent stem cells (iPSCs) differentiation into cardiomyocytes (CMs) via Wnt signaling pathway
• Re-plating cardiomyocytes (CMs) onto fibronectin coating plate

Full setup instructions can be found here.

All code for this project is freely distributed for academic and commercial uses under the MIT license.

Software Requirements

1. Python 3

2. OT2 APP

3. Clone this repository to a local computer.

Authors

• Rita R. Chen - rychen58