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0.4.0

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@TomSmithCGAT TomSmithCGAT released this 09 Mar 16:34
· 614 commits to master since this release

Added functionality:

  • Deduplicating on gene ids ( #44 for motivation):
    The user can now group/dedup according to the gene which the read aligns to. This is useful for single cell RNA-Seq methods such as e.g CEL-Seq where the position of the read on a transcript may be different for reads generated from the same initial molecule. The following options may be used define the gene_id for each read:
    --per-gene
    --gene-transcript-map
    --gene-tag

  • Working with BAM tags (#73, #76, #89):
    UMIs can now be extracted from the BAM tags and_group_ will add a tag to each read describing the read group and UMI. See following options for controlling this behaviour:
    --extract-umi-method
    --umi-tag
    --umi-group-tag

  • Ouput unmapped reads (#78)
    The group command will now output unmapped reads if the --output-unmapped is supplied. These reads will not be assigned to any group.

+ bug fixes for group command (#67, #81) and updated documentation (#77, #79 )