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Expand Up @@ -30,7 +30,7 @@ Thus, the genetic diversity indicators adopted at COP15 by the Global Biodiversi

![](docs/PMNe500_diagram.png)

The Ne 500 indicator measures **the proportion of populations within a species that are of sufficient size to maintain genetic diversity and adaptive potential** within that species. When a population is below a certain size threshold (i.e., Ne 500), genetic diversity loss starts to occur, and at population sizes much smaller than this threshold, genetic diversity loss can be very rapid (Frankham 2021). So it is important to maintain or restore populations above this Ne 500 threshold. The ideal state for this indicator is a value of 1, indicating that all populations are of sufficient size (that each population is above Ne 500). For many species, it is sufficient and appropriate to use census size (Nc: the number of living adults) as a proxy for Ne, with the default threshold translating to Nc = 5000 mature individuals (though see Section [How to estimate population sizes](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/3_Howto_guides_examples/Populations_sizes.html#how-to-estimate-population-sizes). **The Ne 500 indicator is likely the best evidence of genetic status and risk of genetic erosion when DNA sequencing is not available (the case for most species globally)**.
The Ne 500 indicator measures **the proportion of populations within a species that are of sufficient size to maintain genetic diversity and adaptive potential** within that species. When a population is below a certain size threshold (i.e., Ne 500), genetic diversity loss starts to occur, and at population sizes much smaller than this threshold, genetic diversity loss can be very rapid (Frankham 2021). So it is important to maintain or restore populations above this Ne 500 threshold. The ideal state for this indicator is a value of 1, indicating that all populations are of sufficient size (that each population is above Ne 500). For many species, it is sufficient and appropriate to use census size (Nc: the number of living adults) as a proxy for Ne, with the default threshold translating to Nc = 5000 mature individuals (though see Section [How to estimate population sizes](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/3_Howto_guides_examples/Populations_sizes.html#how-to-estimate-population-sizes)). **The Ne 500 indicator is likely the best evidence of genetic status and risk of genetic erosion when DNA sequencing is not available (the case for most species globally)**.

The PM indicator measures **the proportion of populations that still exist compared to the total number of populations that used to occur** (i.e., it is a way of quantifying population extinctions). Each population is presumed to be genetically distinct and locally adapted, and hence loss of any population within a species equates to the loss of genetic diversity and a species’ unique genetic adaptations. Similar to the Ne 500 indicator, the ideal state for this indicator is a value of 1, indicating that no populations have been lost.

Expand All @@ -56,6 +56,7 @@ In December 2022, 196 Parties to the CBD adopted the Kunming-Montreal Global Bio
###### Components of Goal A and Target 4 of the Global Biodiversity Framework that specifically address genetic diversity.



The Ne 500 indicator is directly relevant to **Goal A**, as it informs the health and resilience of species’ populations, their genetic diversity, and the threat of species extinction. Knowledge of species population’s effective size is relevant to **Target 4** as it facilitates active management of species, ex situ breeding programs and informs the conservation efforts and recovery process of species populations following environmental disruption. The Ne 500 indicator is a Headline indicator for Goal A and Target 4.

The complementary indicator for Goal A, the “proportion of populations maintained”, is necessary for maintaining genetic diversity among populations. This indicator is directly relevant to addressing the “between populations” portion of **Target 4**, despite the GBF not making a direct link. Both the Ne 500 and PM indicators complement each other, and **experts agree that both are critical for assessing and monitoring the genetic health of species** (Hoban et al 2020, Hoban et al 2023b). Helpfully, quantifying the PM indicator (understanding the number of populations within a species - both extant and extinct) can be performed simultaneously to quantifying the Ne 500 indicator.
Expand All @@ -74,11 +75,9 @@ The following video offers a short explanation of the genetic diversity indicato

This documentation is dual-licensed under CC BY 4.0. Attribution 4.0 Internationa for the media and all non-software materials and MIT License for the scripts and other provided code [LICENSE](https://raw.githubusercontent.com/CCGenetics/guidelines-genetic-diversity-indicators/main/LICENSE). The repository uses the [Just the Docs](https://just-the-docs.com/) theme.

The documentation was developed by the genetic diversity indicator team.

If you use any of the materials of these guidelines **please cite**:

Mastretta-Yanes, A., Sofía Suárez, Rebecca Jordan, Sean Hoban, Jessica M. da Silva, Luis Castillo-Reina, Myriam Heuertz, Fumiko Ishihama, Viktoria Köppä, Linda Laikre, Joachim Mergeay, Ivan Paz-Vinas, Gernot Segelbacher, Alicia Knapps, Henry Rakoczy, Amelie Weiler & Brenna R. Forester. Guideline materials and documentation for the Genetic Diversity Indicators. https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/
Mastretta-Yanes, A., Sofía Suárez, Rebecca Jordan, Sean Hoban, Jessica M. da Silva, Luis Castillo-Reina, Myriam Heuertz, Fumiko Ishihama, Viktoria Köppä, Linda Laikre, Joachim Mergeay, Ivan Paz-Vinas, Gernot Segelbacher, Alicia Knapps, Henry Rakoczy, Amelie Weiler & Brenna R. Forester. Guideline materials and documentation for the Genetic Diversity Indicators of the monitoring framework for the Kunming-Montreal Global Biodiversity Framework. https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/

## Acknowledgments

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