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add links to koboform and r scripts more clearly
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AliciaMstt authored Apr 13, 2024
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# Quickstart guide to genetic indicators
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Here we summarize the ten steps to estimate the Ne 500 and PM genetic diversity indicators. Please refer to the [Species list](https://github.com/AliciaMstt/guidelines-genetic-diversity-indicators/blob/main/docs/4_Species_list/Species_list.md#species-list), [How to guides](https://github.com/AliciaMstt/guidelines-genetic-diversity-indicators/blob/main/docs/3_Howto_guides_examples/Howto_guides_examples.md#how-to-guides--examples), and [Calculations and reporting](https://github.com/AliciaMstt/guidelines-genetic-diversity-indicators/blob/main/docs/6_Calculations_and_reporting/Calculations_and_reporting.md#calculations-and-reporting) sections of these guidelines for more detailed advice.
Here we summarize the ten steps to estimate and use the Ne 500 and PM genetic diversity indicators. Please refer to the [Species list](https://github.com/AliciaMstt/guidelines-genetic-diversity-indicators/blob/main/docs/4_Species_list/Species_list.md#species-list), [How to guides](https://github.com/AliciaMstt/guidelines-genetic-diversity-indicators/blob/main/docs/3_Howto_guides_examples/Howto_guides_examples.md#how-to-guides--examples), and [Calculations and reporting](https://github.com/AliciaMstt/guidelines-genetic-diversity-indicators/blob/main/docs/6_Calculations_and_reporting/Calculations_and_reporting.md#calculations-and-reporting) sections of these guidelines for more detailed advice.

Notice that we have made available a Kobotoolbox form that you can use to gather data and metadata from steps 2-5, and a series of R scripts to perform the calculations from steps 5-8.
Notice that we have made available a [Kobotoolbox web-form that you can use to gather data and metadata](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/5_Data_collection/Web_tool.html) from steps 2-5, and a series of [R scripts](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/6_Calculations_and_reporting/R_scripts.html) to perform the calculations from steps 5-8.


## Table of contents
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## Gather data

Notice that we have made available a [Kobotoolbox web-form that you can use to gather data and metadata](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/5_Data_collection/Web_tool.html).

### Step 2: Define population boundaries in geographic space

For each focal species it is first necessary to define ‘populations’. Many local and national biodiversity monitoring programs (e.g. at species or ecosystem level) have already defined populations, or it may be straightforward to define them based on geographic isolation, occupying distinct habitats or ecoregions, association with a geographic feature like a mountain range or lake, etc. The goal is to have a discrete count, e.g. 4 populations. However, the indicator can accommodate uncertainty, such that different numbers of populations may be assumed. Full guidance on defining populations for a wide variety of organisms are provided in the [How to define populations](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/3_Howto_guides_examples/Howto_define_populations.html#how-to-define-populations) section. See also. [What is a population?](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/2_Theoretical_background/What-is-a-population.html#what-is-a-population-a-first-simple-answer) in the background section.
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To convert Nc to Ne it is important to choose a ratio of effective-to-census size and then multiplying the population’s census size by this ratio to obtain the population’s effective size. As mentioned in the [Ne 500 indicator background](https://github.com/AliciaMstt/guidelines-genetic-diversity-indicators/blob/main/docs/2_Theoretical_background/Ne-500.md#ne-500-indicator) the default ratio, which is slightly conservative, is 1/10th or 0.1 (thus the minimum Nc would be 5000). Alternatively, a taxon-specific ratio can be obtained several ways. See How to estimate population sizes? in the How to guides. To incorporate uncertainty in calculations, the calculation can be repeated using multiple Ne/Nc ratios. But it is acceptable and useful to use the well-recognized 0.1 ratio. See section [Calculations and reporting](https://github.com/AliciaMstt/guidelines-genetic-diversity-indicators/blob/main/docs/6_Calculations_and_reporting/Calculations_and_reporting.md#calculations-and-reporting) for details, [equations](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/6_Calculations_and_reporting/Equations.html#equations-and-example-calculations) and [R scripts](https://ccgenetics.github.io/guidelines-genetic-diversity-indicators/docs/6_Calculations_and_reporting/R_scripts.html#r-scripts-for-calculation-and-reporting) to help with the calculations.


## Use data to calculate indicators

### Step 6. Calculate the proportion of maintained populations (PM indicator)
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