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feat: add remaining test datasets from encode #215

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Nov 27, 2024
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1 change: 1 addition & 0 deletions CHANGELOG.md
Original file line number Diff line number Diff line change
Expand Up @@ -2,6 +2,7 @@

- The CHAMPAGNE nextflow workflow now has a version entry in `nextflow.config`, in compliance with nf-core. (#213, @kelly-sovacool)
- Pool input (control) reads of the same sample name by default. Any inputs that should not be pooled must have different sample names in the samplesheet. (#214, @kelly-sovacool)
- Add histone samples to the `test_human` dataset. (#215, @kelly-sovacool)

## CHAMPAGNE 0.4.0

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8 changes: 8 additions & 0 deletions assets/samplesheet_human.csv
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Expand Up @@ -11,3 +11,11 @@ fastq_1,fastq_2,sample,rep,antibody,control
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/SRR14638304.fastq.gz,,A549_JUN_INPUT,1,,
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/SRR14638305.fastq.gz,,A549_JUN_INPUT,2,,
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/SRR14638306.fastq.gz,,A549_JUN_INPUT,3,,
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/ENCFF391TRF_ENCFF822KCO.fastq.gz,,BLaER1_H3K4me3,1,H3K4me3,BLaER1_INPUT
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/ENCFF989TVW.fastq.gz,,BLaER1_H3K4me3,2,H3K4me3,BLaER1_INPUT
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/ENCFF097DJJ_ENCFF815UIL.fastq.gz,,BLaER1_H3K27ac,1,H3K27ac,BLaER1_INPUT
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/ENCFF161DOP.fastq.gz,,BLaER1_H3K27ac,2,H3K27ac,BLaER1_INPUT
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/ENCFF963ONJ.fastq.gz,,BLaER1_H3K36me3,1,H3K36me3,BLaER1_INPUT
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/ENCFF686GPG.fastq.gz,,BLaER1_H3K36me3,2,H3K36me3,BLaER1_INPUT
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/ENCFF259JCU.fastq.gz,,BLaER1_INPUT,1,,
/data/CCBR_Pipeliner/testdata/CHAMPAGNE/human/ENCFF622MZI.fastq.gz,,BLaER1_INPUT,2,,
3 changes: 1 addition & 2 deletions main.nf
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Expand Up @@ -72,9 +72,8 @@ workflow {
}

workflow CHIPSEQ {
INPUT_CHECK(file(params.input, checkIfExists: true), params.seq_center, contrast_sheet)
raw_fastqs = INPUT_CHECK(file(params.input, checkIfExists: true), params.seq_center, contrast_sheet).reads

INPUT_CHECK.out.reads.set { raw_fastqs }
CUTADAPT(raw_fastqs).reads | POOL_INPUTS
trimmed_fastqs = POOL_INPUTS.out.reads

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