Out of memory error when processing fluorescent traces

[gg9761]

I have been trying to run a large data set through process fluorescent traces (243 cells, 25-30 min timelapse), but keep getting the out of memory error in matlab. About 5 mins into step 3.1 estimating noise model, the RAM is maxed out (see attached picture). The computer I'm using has 48GB RAM, I have also tried using another computer with more RAM and get similar out of memory issues in matlab.
Any suggestions on how to solve this problem would be much appreciated.

[ElTinmar]

Hi, this is caused by the density estimation procedure when you have outliers in the data. This could be due to weird/abrupt changes in DF/F values in the data caused by movements/ artifacts in the time series. More specifically, the programs tries to create a gaussian filter whose size is too large for the memory, because the variable binSpread is too big. One workaround is too check the DF/F values and remove outliers (DF/F >5 or more is suspicious)

[sebromano]

ElTinmar is right, these errors are the result of imaging frames with movement artifacts that lead to large deviations in DF/F. Right now the solution is to identify these frames and remove them. We are working on a solution to handle these situations.