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When using the "smooth slow dynamics" option for baseline estimation, I can see that the amplitude of calcium transients increases as time goes on across pretty much all cells (see image below). When I look at my raw traces, it's clear that the baseline fluorescence is gradually falling over the course of the ten minute video (I'm guessing it's a bit of photobleaching), which is probably causing an artifact where the same amplitude of raw transient now looks bigger in the dF/F trace because the baseline is lower. Is there any way to tweak the code to account for this?
Thanks!
(BTW if anyone would like to use the script I made to only call the increasing portion of the calcium transient significant, I posted it here: https://github.com/slceto/CalFDR)
The text was updated successfully, but these errors were encountered:
When using the "smooth slow dynamics" option for baseline estimation, I can see that the amplitude of calcium transients increases as time goes on across pretty much all cells (see image below). When I look at my raw traces, it's clear that the baseline fluorescence is gradually falling over the course of the ten minute video (I'm guessing it's a bit of photobleaching), which is probably causing an artifact where the same amplitude of raw transient now looks bigger in the dF/F trace because the baseline is lower. Is there any way to tweak the code to account for this?
Thanks!
(BTW if anyone would like to use the script I made to only call the increasing portion of the calcium transient significant, I posted it here: https://github.com/slceto/CalFDR)
The text was updated successfully, but these errors were encountered: