From 2444e584c4e24c39ffda16c02dd11f5184b50711 Mon Sep 17 00:00:00 2001 From: Diego Diez Date: Fri, 2 Jun 2023 01:25:46 +0900 Subject: [PATCH] Update scrna_seq.md (#203) Correct MinIO -> MinION. --- jupyter-book/introduction/scrna_seq.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/jupyter-book/introduction/scrna_seq.md b/jupyter-book/introduction/scrna_seq.md index 7bb9d59e..4fb68643 100644 --- a/jupyter-book/introduction/scrna_seq.md +++ b/jupyter-book/introduction/scrna_seq.md @@ -86,7 +86,7 @@ First, long-read sequencing, which describes the ability to obtain nucleotide fr Pacific Biosciences' (PacBio) introduced zero-mode waveguide (ZMW) sequencing in 2010, which uses so-called nanoholes containing a single DNA polymerase. This allows incorporation of any single nucleotide to be directly observed by detectors attached below the nanoholes. Each type of nucleotides is labeled with a specific fluorescent dye that emits fluorescent signals during the incorporation process, which are subsequently measured as sequence readout. Reads obtained from PacBio sequencers are usually of 8 to 15 kilobases (kb), with possibilities for up to 70kb. -Oxford Nanopore Technologies' introduced the GridION in 2012. The GridION and its successors MinIO and Flongle are portable sequencers for DNA and RNA sequencing which produce reads of more than 2 Mb. Notably, such a sequencing device even fits into a single human hand. Oxford Nanopore sequencers observe changes in the electrical current that occur when nucleic acids pass through protein nanopores, to identify the nucleotide sequence{cite}`Jain2016`. +Oxford Nanopore Technologies' introduced the GridION in 2012. The GridION and its successors MinION and Flongle are portable sequencers for DNA and RNA sequencing which produce reads of more than 2 Mb. Notably, such a sequencing device even fits into a single human hand. Oxford Nanopore sequencers observe changes in the electrical current that occur when nucleic acids pass through protein nanopores, to identify the nucleotide sequence{cite}`Jain2016`. Strengths: