decidualised endometrial stromal cells in proliferative phase ----'Mapping the temporal and spatial dynamics of the human endometrium in vivo and in vitro'

[Chris-lang478]

Dear authors:
Thanks for your great work!
I just downloaded processed matrices can be accessed and downloaded from [www.reproductivecellatlas.org],howerver i found 860 decidualised endometrial stromal cells in proliferative phase of menstrual period. I am confused about these cells,should i exclude them before the next step analysis?
Hope for your reply! Thanks a lot!

[luzgaral]

Hi,

Thank you for your interest.

Please note that cell annotation is done based on unbiased clustering, which artificially categorises data into two groups. Instead, decidualization is a continuum process so it is not unexpected to have proliferative phase cells falling in the boundaries of decidualised stromal.

Also bear in mind that proliferative/secretory classification of the samples is done by histology (us) or based on menstrual cycle day (Wang et al). Because none is tracking actual ovulation with LH-surge or progesterone blood levels, it may be challenging to distinguish samples transitioning from Proliferative to Secretory.

Having said all this, filtering these cells is up to you and your research question.

Hope this helps.

Best

Luz

[Chris-lang478]

Thank you so much.