forked from evidenceontology/evidenceontology
-
Notifications
You must be signed in to change notification settings - Fork 0
/
Copy pathevidence_ontology.obo
2266 lines (1995 loc) · 107 KB
/
evidence_ontology.obo
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
801
802
803
804
805
806
807
808
809
810
811
812
813
814
815
816
817
818
819
820
821
822
823
824
825
826
827
828
829
830
831
832
833
834
835
836
837
838
839
840
841
842
843
844
845
846
847
848
849
850
851
852
853
854
855
856
857
858
859
860
861
862
863
864
865
866
867
868
869
870
871
872
873
874
875
876
877
878
879
880
881
882
883
884
885
886
887
888
889
890
891
892
893
894
895
896
897
898
899
900
901
902
903
904
905
906
907
908
909
910
911
912
913
914
915
916
917
918
919
920
921
922
923
924
925
926
927
928
929
930
931
932
933
934
935
936
937
938
939
940
941
942
943
944
945
946
947
948
949
950
951
952
953
954
955
956
957
958
959
960
961
962
963
964
965
966
967
968
969
970
971
972
973
974
975
976
977
978
979
980
981
982
983
984
985
986
987
988
989
990
991
992
993
994
995
996
997
998
999
1000
format-version: 1.2
data-version: 2.00
date: 25:03:2012 23:17
saved-by: mchibucos
auto-generated-by: OBO-Edit 2.1.1-beta7
default-namespace: evidence_code2.obo
ontology: eco
[Term]
id: ECO:0000000
name: evidence
def: "A type of information that is used to support an assertion." [ECO:MCC]
synonym: "evidence code" RELATED []
synonym: "evidence_code" RELATED []
[Term]
id: ECO:0000001
name: inference from background scientific knowledge
def: "A curator inference that links the current annotation to a different evidence-based annotation via background knowledge of the curator." [ECO:go, ECO:MCC]
synonym: "IC" EXACT [GO:IC]
synonym: "inferred by curator" EXACT [GO:IC]
is_a: ECO:0000205 ! curator inference
[Term]
id: ECO:0000002
name: direct assay evidence
def: "Experimental evidence that is based on direct measurement of some aspect of a biological feature." [ECO:MCC, GO:IDA]
is_a: ECO:0000006 ! experimental evidence
[Term]
id: ECO:0000003
name: reconstitution assay evidence
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000004
name: cell fractionation evidence
def: "Used when an annotation is made based on separation of subcellular components based on their physical properties, such as density in sucrose density gradients. This evidence is used mostly for annotations to the cellular component ontology." [TAIR:TED]
synonym: "IDA: cell fractionation" RELATED []
is_a: ECO:0000100 ! fractionation evidence
[Term]
id: ECO:0000005
name: enzyme assay evidence
def: "Used when an annotation is made based on assays that determine the catalytic activity of enzymes." [TAIR:TED]
synonym: "IDA: enzyme assays" RELATED []
is_a: ECO:0000039 ! protein assay evidence
[Term]
id: ECO:0000006
name: experimental evidence
def: "An evidence type that is based on the results of a laboratory assay." [ECO:MCC, GOC:ecd]
synonym: "EXP" EXACT [GO:EXP]
synonym: "inferred from experiment" EXACT [GO:EXP]
is_a: ECO:0000000 ! evidence
[Term]
id: ECO:0000007
name: immunofluorescence evidence
def: "Used when an annotation is made based on methods that detect the presence of macromolecules, proteins, and compounds by the use of a fluorescent-labeled antibody." [TAIR:TED]
synonym: "IDA: immunofluorescence" RELATED []
is_a: ECO:0000040 ! immunological assay evidence
[Term]
id: ECO:0000008
name: expression pattern evidence
def: "Experimental evidence that is based on characterization of gene expression." [ECO:MCC, GO:IEP]
comment: Use this evidence type when the annotation is inferred from the timing or location of expression of a gene. It may be difficult to determine whether the expression pattern truly indicates that a gene plays a role in a given process.
synonym: "IEP" EXACT [GO:IEP]
synonym: "inferred from expression pattern" EXACT [GO:IEP]
is_a: ECO:0000006 ! experimental evidence
[Term]
id: ECO:0000009
name: transcript expression evidence
is_a: ECO:0000008 ! expression pattern evidence
[Term]
id: ECO:0000010
name: protein expression evidence
is_a: ECO:0000008 ! expression pattern evidence
is_a: ECO:0000039 ! protein assay evidence
[Term]
id: ECO:0000011
name: genetic interaction evidence
xref: TAIR:TED\:0000006
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000012
name: functional complementation evidence
def: "Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a mutant background in the organism of the gene's origin or a heterologous organism with a mutation in the homologous gene." [TAIR:TED]
synonym: "IGI: functional complementation" RELATED []
is_a: ECO:0000011 ! genetic interaction evidence
[Term]
id: ECO:0000013
name: transgenic rescue experiment evidence
is_a: ECO:0000012 ! functional complementation evidence
[Term]
id: ECO:0000015
name: mutant phenotype evidence
def: "The IMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene. The IMP code is used for cases where one allele may be designated 'wild-type' and another as 'mutant'. It is also used in cases where allelic variation occurs naturally and no specific allele is designated as wild-type or mutant. Caution should be used when making annotations from gain-of-function mutations as it may be difficult to infer a gene's normal function from a gain of function mutation, although it is sometimes possible." [GO:IMP]
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000016
name: loss-of-function mutant phenotype evidence
is_a: ECO:0000015 ! mutant phenotype evidence
[Term]
id: ECO:0000017
name: ectopic expression evidence
def: "Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress or ectopically express the gene product in question." [TAIR:TED]
synonym: "IMP: analysis of overexpression/ectopic expression phenotype" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000018
name: anti-sense experiment evidence
def: "Used when an annotation is based on a phenotype observed when expressing an anti-sense version of a gene product in a wild-type (for that gene product) background." [TAIR:TED]
synonym: "IMP: anti-sense experiments" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000019
name: RNAi evidence
def: "Inference based on a phenotype observed when expressing an RNAi construct that includes a fragment of the gene product in a wild-type (for that gene product) background. A double-stranded RNA specifically suppresses the expression of the gene bearing its complementary sequence." [TAIR:TED]
synonym: "IMP: RNAi experiment" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000020
name: specific protein inhibition experiment evidence
is_a: ECO:0000039 ! protein assay evidence
[Term]
id: ECO:0000021
name: physical interaction evidence
def: "Experimental evidence that is based on characterization of an interaction between a gene product and another molecule." [ECO:MCC]
comment: Molecules interacted with might include protein, nucleic acid, ion, or complex.
synonym: "inferred from physical interaction" RELATED [GOECO:IPI]
synonym: "IPI" RELATED [GOECO:IPI]
xref: GOECO:IPI "inferred from physical interaction"
is_a: ECO:0000006 ! experimental evidence
[Term]
id: ECO:0000022
name: co-purification evidence
def: "Used in component annotations when a subunit is isolated as part of purification of its larger complex." [TAIR:TED]
synonym: "IPI: co-purification" RELATED []
is_a: ECO:0000021 ! physical interaction evidence
[Term]
id: ECO:0000023
name: ligand binding evidence
is_a: ECO:0000021 ! physical interaction evidence
[Term]
id: ECO:0000024
name: protein binding evidence
is_a: ECO:0000023 ! ligand binding evidence
is_a: ECO:0000039 ! protein assay evidence
[Term]
id: ECO:0000025
name: hybrid interaction evidence
is_a: ECO:0000023 ! ligand binding evidence
[Term]
id: ECO:0000026
name: nucleic acid hybridization evidence
is_a: ECO:0000073 ! experimental genomic evidence
[Term]
id: ECO:0000027
name: structural similarity evidence
def: "Used when an annotation is made based on the structural similarity of the annotated gene or gene product to a single other gene or group of genes. In the case of a single gene, an accession for the related gene's sequence is entered in the evidence_with field." [TAIR:TED]
synonym: "inferred from sequence or structural similarity" BROAD [GO:ISS]
synonym: "ISS" BROAD [GO:ISS]
is_a: ECO:0000057 ! phenotypic similarity evidence
[Term]
id: ECO:0000028
name: motif similarity evidence
def: "Used when an annotation is made based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence." [TAIR:TED]
synonym: "ISS: recognized domains" RELATED []
is_a: ECO:0000044 ! sequence similarity evidence
[Term]
id: ECO:0000029
name: match to InterPro signature evidence
is_a: ECO:0000202 ! match to sequence model evidence
[Term]
id: ECO:0000030
name: BLAST evidence used in manual assertion
def: "A type of BLAST evidence that is used in a manual assertion." [ECO:MCC]
synonym: "curated BLAST analysis" RELATED []
is_a: ECO:0000206 ! BLAST evidence
intersection_of: ECO:0000206 ! BLAST evidence
intersection_of: used_in ECO:0000218 ! manual assertion
[Term]
id: ECO:0000031
name: protein BLAST evidence used in manual assertion
def: "Protein BLAST evidence that is used in a manual assertion." [ECO:MCC]
synonym: "curated protein BLAST analysis" RELATED []
xref: GO_REF:0000012 "Pairwise alignment (TIGR)"
xref: GO_REF:0000018 "dictyBase 'Inferred from Electronic Annotation (BLAST method)'"
xref: GO_REF:0000027 "BLAST search criteria for ISS assignment in PAMGO_GAT"
is_a: ECO:0000208 ! protein BLAST evidence
intersection_of: ECO:0000208 ! protein BLAST evidence
intersection_of: used_in ECO:0000218 ! manual assertion
[Term]
id: ECO:0000032
name: nucleotide BLAST evidence used in manual assertion
def: "Nucleotide BLAST evidence that is used in a manual assertion." [ECO:MCC]
synonym: "curated nucleic acid BLAST analysis" RELATED []
is_a: ECO:0000207 ! nucleotide BLAST evidence
intersection_of: ECO:0000207 ! nucleotide BLAST evidence
intersection_of: used_in ECO:0000218 ! manual assertion
[Term]
id: ECO:0000033
name: traceable author statement
def: "An author statement that is based on a cited reference." [ECO:MCC, GO:TAS]
comment: The traceable author statement (TAS) evidence code covers author statements that are attributed to a cited source. Typically this type of information comes from review articles. Material from the introductions and discussion sections of non-review papers may also be suitable if another reference is cited as the source of experimental work or analysis. When annotating with this code the curator should use caution and be aware that authors often cite papers dealing with experiments that were performed in organisms different from the one being discussed in the paper at hand. Thus a problem with the TAS code is that it may turn out from following up the references in the paper that no experiments were performed on the gene in the organism actually being characterized in the primary paper. For this reason we recommend (when time and resources allow) that curators track down the cited paper and annotate directly from the experimental paper using the appropriate experimental evidence code. When this is not possible and it is necessary to annotate from reviews, the TAS code is the appropriate code to use for statements that are associated with a cited reference. Once an annotation has been made to a given term using an experimental evidence code, we recommend removing any annotations made to the same term using the TAS evidence code.
is_a: ECO:0000204 ! author statement
[Term]
id: ECO:0000034
name: non-traceable author statement
def: "An author statement that is not associated with results presented or a cited reference." [ECO:MCC]
comment: The non-traceable author statement evidence code should be used in all cases where the author makes a statement that a curator wants to capture but for which there are neither results presented nor a specific reference cited in the source used to make the annotation. The source of the information may be peer reviewed papers, textbooks, database records or vouchered specimens.
is_a: ECO:0000204 ! author statement
[Term]
id: ECO:0000035
name: no biological data found
def: "A curator inference that states that a search found no information about a biological feature." [ECO:MCC, GO:ND]
synonym: "ND" EXACT [GO:ND]
synonym: "no biological data available" EXACT [GO:ND]
is_a: ECO:0000205 ! curator inference
[Term]
id: ECO:0000037
name: not_recorded
def: "An evidence that reflects an annotation was made before curators began tracking evidence types." [ECO:MCC, GO:NR]
comment: The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations.
synonym: "not recorded" EXACT [GO:NR]
synonym: "NR" EXACT [GO:NR]
is_obsolete: true
[Term]
id: ECO:0000038
name: transient rescue experiment evidence
is_a: ECO:0000012 ! functional complementation evidence
[Term]
id: ECO:0000039
name: protein assay evidence
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000040
name: immunological assay evidence
is_a: ECO:0000023 ! ligand binding evidence
is_a: ECO:0000039 ! protein assay evidence
[Term]
id: ECO:0000041
name: similarity evidence
def: "An evidence type that is based on comparing likeness of distinct biological entities." [ECO:MCC, PhenoScape:IS]
synonym: "inferred from similarity" RELATED []
synonym: "IS" EXACT []
is_a: ECO:0000000 ! evidence
[Term]
id: ECO:0000042
name: gain-of-function mutant phenotype evidence
is_a: ECO:0000015 ! mutant phenotype evidence
[Term]
id: ECO:0000044
name: sequence similarity evidence
def: "A type of similarity based on biomolecular sequence." [ECO:MCC, TAIR:TED]
comment: A sequence similarity analysis may involve a gene or a gene product, and it could be based on similarity to a single other gene or to a group of other genes.
synonym: "inferred from sequence or structural similarity" BROAD [GO:ISS]
synonym: "inferred from sequence similarity" EXACT []
synonym: "ISS" BROAD [GO:ISS]
is_a: ECO:0000041 ! similarity evidence
[Term]
id: ECO:0000045
name: spatial pattern of protein expression evidence
is_a: ECO:0000010 ! protein expression evidence
[Term]
id: ECO:0000046
name: protein expression level evidence
is_a: ECO:0000010 ! protein expression evidence
[Term]
id: ECO:0000047
name: spatial pattern of transcript expression evidence
is_a: ECO:0000009 ! transcript expression evidence
[Term]
id: ECO:0000048
name: transcript expression level evidence
is_a: ECO:0000009 ! transcript expression evidence
[Term]
id: ECO:0000049
name: reporter gene assay evidence
def: "Used when an annotation is made on the basis of the expression pattern of a reporter gene. The reporter gene may consist of the native promoter of the gene in question that drives the expression of a readily assayable gene product such as GUS or GFP. The reporter gene may also be a marker of a particular type of physiological response." [TAIR:TED]
synonym: "IEP: expression of a reporter gene" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000050
name: voucher specimen analysis evidence
def: "Used when an annotation is made on the basis of for a phenotype description for a species or higher level group that given by an author who explicitly references an observation of a voucher specimen(s). Voucher specimens are defined as those specimens with permanent museum catalog numbers. Thus it would be possible for a person to examine this particular specimen and observe the annotated phenotype." [PhenoScape:DOA]
synonym: "IVS" EXACT []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000051
name: genetic similarity evidence
def: "A type of similarity based on genotype without respect to expression." [ECO:MCC, PhenoScape:IGTS]
comment: A genetic similarity analysis might consider genetic markers, polymorphisms, alleles, or other characteristics sometimes considered as part of the field of traditional genetics. Although an attempt has been made to treat as distinct the concepts of "genetic", "genotypic", "genomic", and "sequence", there is considerable overlap in usage throughout the field of biology.
synonym: "IGTS" EXACT []
synonym: "inferred from genetic similarity" RELATED []
is_a: ECO:0000041 ! similarity evidence
[Term]
id: ECO:0000052
name: suppressor/enhancer interaction evidence
synonym: "IGI: 'traditional' genetic interactions (e.g. suppressors, synthetic lethals)" RELATED []
xref: TAIR:TED\:0000063
is_a: ECO:0000011 ! genetic interaction evidence
[Term]
id: ECO:0000053
name: computational combinatorial evidence
def: "A type of combinatorial analysis where data are combined and evaluated by an algorithm." [ECO:go, ECO:MCC]
comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments.
xref: TAIR:TED\:0000004
is_a: ECO:0000212 ! combinatorial evidence
[Term]
id: ECO:0000054
name: double mutant phenotype evidence
def: "Used when an annotation is made based on the phenotype of double mutant organism that contains a mutation in the gene product of interest in addition to a second mutation at an unrelated locus." [TAIT:TED]
synonym: "IGI: double mutant analysis" RELATED []
is_a: ECO:0000011 ! genetic interaction evidence
[Term]
id: ECO:0000055
name: array experiment evidence
is_a: ECO:0000008 ! expression pattern evidence
[Term]
id: ECO:0000056
name: epistatic interaction evidence
def: "Used when an annotation is based on results of epistatis analysis of an allele of the gene in question and a mutant allele at another locus." [TAIR:TED]
synonym: "IGI: epistatic interactions" RELATED []
is_a: ECO:0000011 ! genetic interaction evidence
[Term]
id: ECO:0000057
name: phenotypic similarity evidence
def: "A type of similarity based on the expression of a genotype in an environment." [ECO:MCC, PhenoScape:IPTS]
comment: Phenotype is defined as the outcome of the expression of a genotype in a given environment. A comparison might involve whole organisms or sub-parts of organisms.
synonym: "inferred from phenotypic similarity" RELATED []
synonym: "IPTS" EXACT []
is_a: ECO:0000041 ! similarity evidence
[Term]
id: ECO:0000058
name: expression microarray evidence
is_a: ECO:0000055 ! array experiment evidence
[Term]
id: ECO:0000059
name: experimental phenotypic evidence
alt_id: ECO:0000014
def: "Experimental evidence that is based on the expression of a genotype in an environment." [ECO:MCC]
synonym: "inferred from phenotype" RELATED []
is_a: ECO:0000006 ! experimental evidence
[Term]
id: ECO:0000060
name: positional similarity evidence
def: "Used when an annotation is made based on the similarity of the location and or arrangement of structures." [PhenoScape:IPS]
synonym: "IPS" EXACT []
is_a: ECO:0000057 ! phenotypic similarity evidence
[Term]
id: ECO:0000061
name: quantitative trait analysis evidence
def: "Used when an annotation is made for a gene product that has not been cloned but is associated with a quantitative trait locus." [TAIR:tn]
synonym: "IGI: quantitative trait analysis" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000062
name: genomic microarray evidence
is_a: ECO:0000055 ! array experiment evidence
[Term]
id: ECO:0000063
name: compositional similarity evidence
def: "Used when an annotation is made based on the similarity of the histological makeup of structures." [PhenoScape:ICS]
synonym: "ICS" EXACT []
is_a: ECO:0000057 ! phenotypic similarity evidence
[Term]
id: ECO:0000064
name: functional complementation in heterologous system evidence
def: "Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a heterologous organism with a mutation in the homologous gene." [TAIR:TED]
synonym: "IGI: functional complementation in heterologous system" RELATED []
is_a: ECO:0000012 ! functional complementation evidence
[Term]
id: ECO:0000065
name: CpG island microarray evidence
is_a: ECO:0000055 ! array experiment evidence
[Term]
id: ECO:0000066
name: yeast one-hybrid evidence
def: "Used when an annotation is based on the interaction of a protein with a DNA fragment in a yeast one-hybrid assay. The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene." [TAIR:TED]
synonym: "IPI: yeast one-hybrid assay" RELATED []
is_a: ECO:0000025 ! hybrid interaction evidence
[Term]
id: ECO:0000067
name: developmental similarity evidence
def: "Used when an annotation is made based on the similarity of embryological and/or post-embryonic origin of structures." [PhenoScape:IDS]
synonym: "IDS" EXACT []
is_a: ECO:0000057 ! phenotypic similarity evidence
[Term]
id: ECO:0000068
name: yeast 2-hybrid evidence
def: "Used when an annotation is based on the interaction between two proteins in a yeast two-hybrid assay. The assay involves screening a trap-tagged library with a bait-tagged vector. The library is tagged with a DNA-binding domain (trap) and the bait is tagged with a transcriptional activation domain. Interaction between a library-trap protein and the bait results in activation of a yeast reporter gene whose transcription can be experimentally assayed." [TAIR:TED]
synonym: "IPI: yeast two-hybrid assay" RELATED []
is_a: ECO:0000025 ! hybrid interaction evidence
[Term]
id: ECO:0000069
name: differential methylation hybridization evidence
is_a: ECO:0000026 ! nucleic acid hybridization evidence
[Term]
id: ECO:0000070
name: co-immunoprecipitation evidence
def: "Used when an annotation is based on the interaction of one gene product with another in a co-immunoprecipitation assay (two or more proteins are precipitated when treated with an antibody specific to a single protein). The interacting protein is referenced in the evidence_with column." [TAIR:TED]
synonym: "IPI: co-immunoprecipitation" RELATED []
is_a: ECO:0000085 ! immunoprecipitation evidence
[Term]
id: ECO:0000071
name: morphological similarity evidence
def: "Used when an annotation is made based on the similarity of the shape, structure or overall configuration of structures." [PhenoScape:IMS]
synonym: "IMS" EXACT []
is_a: ECO:0000057 ! phenotypic similarity evidence
[Term]
id: ECO:0000072
name: Sos-recruitment assay evidence
def: "Used when an annotation is based on the interaction of two proteins in a Sos-recruitment assay. The assay is a genetic screening system that detects protein-protein interactions and is not based on a transcriptional readout." [TAIR:TED]
synonym: "IPI: Sos-recruitment assay" RELATED []
is_a: ECO:0000021 ! physical interaction evidence
[Term]
id: ECO:0000073
name: experimental genomic evidence
def: "Experimental evidence that characterizes an attribute of the genome underlying a gene product." [ECO:MCC]
synonym: "inferred from genomic analysis" EXACT []
is_a: ECO:0000006 ! experimental evidence
[Term]
id: ECO:0000074
name: split-ubiquitin assay evidence
def: "Used when an annotation is based on the interaction of two proteins in a split-ubiquitin assay. The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquiting (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough together to reconstitute split-ubiquitin, resulting in the release of the reporter protein by the action of the UBPs." [TAIR:TED]
synonym: "IPI: split-ubiquitin assay" RELATED []
is_a: ECO:0000021 ! physical interaction evidence
[Term]
id: ECO:0000075
name: gene expression similarity evidence
def: "Used when an annotation is made based on the similarity of expression of genes in structures." [PhenoScape:IGES]
synonym: "IGES" EXACT []
is_a: ECO:0000057 ! phenotypic similarity evidence
[Term]
id: ECO:0000076
name: far-Western evidence
def: "Used when an annotation is based on the interaction of protein with another in a far-Western assay. The assay involves separating target proteins on an SDS-PAGE gel, blotting to a membrane, hybridization with a protein probe and visualization using a probe-directed antibody. The interacting protein is referenced in the evidence_with column." [TAIR:TED]
synonym: "IPI: far-Western analysis" RELATED []
is_a: ECO:0000021 ! physical interaction evidence
[Term]
id: ECO:0000077
name: methylation-specific polymerase chain reaction evidence
synonym: "methylation-specific PCR evidence" EXACT []
is_a: ECO:0000082 ! polymerase chain reaction evidence
[Term]
id: ECO:0000078
name: Southern hybridization evidence
def: "Used when an annotation is based on the hybridization of a probe derived from the gene in question with a gene of known function/process/component. Can also be used when a probe derived from a gene of known function/process/component hybridizes with the gene in question." [TAIR:TED]
synonym: "ISS: Southern blotting" RELATED []
is_a: ECO:0000026 ! nucleic acid hybridization evidence
[Term]
id: ECO:0000079
name: affinity chromatography evidence
def: "Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent." [TAIR:TED]
synonym: "IDA: affinity chromatography" RELATED []
is_a: ECO:0000023 ! ligand binding evidence
[Term]
id: ECO:0000080
name: phylogenetic evidence
def: "A type of similarity that indicates common ancestry." [ECO:MCC, PhenoScape:IP]
synonym: "IP" EXACT [PhenoScape:IP]
is_a: ECO:0000041 ! similarity evidence
[Term]
id: ECO:0000081
name: targeting sequence prediction evidence
def: "Used when a component annotation is made based on the presence of a targeting sequence in a protein's primary sequence. The presence of the targeting sequence may be detected by computational prediction and/or the author's manual examination of the sequence." [TAIR:TED]
synonym: "ISS: targeting sequence prediction" RELATED []
is_a: ECO:0000028 ! motif similarity evidence
[Term]
id: ECO:0000082
name: polymerase chain reaction evidence
synonym: "PCR evidence" EXACT []
is_a: ECO:0000073 ! experimental genomic evidence
[Term]
id: ECO:0000083
name: transmembrane domain prediction evidence
def: "Used when a component annotation is made based on the presence of one or more transmembrane domains in a protein's primary sequence. The presence of the transmembrane domains may be detected by computational prediction and/or the author's manual examination of the sequence." [TAIR:TED]
synonym: "ISS: transmembrane domain prediction" RELATED []
is_a: ECO:0000028 ! motif similarity evidence
[Term]
id: ECO:0000084
name: gene neighbors evidence
def: "Evidence that supports the identity of a gene product on the basis of the identity of neighboring genes." [ECO:MCC, GOC:mg]
comment: Genomic cluster analyses include synteny and operon structure.
synonym: "ICL" RELATED []
synonym: "inferred from genome cluster" EXACT []
xref: GO_REF:0000025 "Operon structure as IGC evidence"
is_a: ECO:0000177 ! genomic context evidence
created_by: mgiglio
creation_date: 2009-03-20T11:55:18Z
[Term]
id: ECO:0000085
name: immunoprecipitation evidence
def: "Used when an annotation is made based on a method that precipitates a multivalent antigen by a bivalent antibody." [TAIR:TED]
synonym: "IDA: immunoprecipitation" RELATED []
is_a: ECO:0000040 ! immunological assay evidence
[Term]
id: ECO:0000086
name: intermethylated site amplification evidence
is_a: ECO:0000073 ! experimental genomic evidence
[Term]
id: ECO:0000087
name: immunolocalization evidence
def: "Used when an annotation is made based on methods that detect the presence of macromolecules, proteins, and compounds by the use of antibodies." [TAIR:TED]
synonym: "IDA: immunolocalization" RELATED []
is_a: ECO:0000040 ! immunological assay evidence
is_a: ECO:0000122 ! protein localization evidence
[Term]
id: ECO:0000088
name: biological system reconstruction
def: "Evidence based on reconstruction of a biological system that is based on an existing experimentally supported model of that biological system." [ECO:MCC, GOC:mg]
comment: The biological system in question might be a multi-step process or a physical complex comprising several components. A common basis for system reconstruction is orthology, where all of the components of an experimentally characterized complex exist in one organism; if a second organism contains orthologs of all components, then the existence of the complex is inferred. In the case of a genome-wide analysis, a gene product is said to fulfill a necessary function for a pathway or complex when, with all of the other necessary functions or components also present, the pathway or complex is reconstructed.
synonym: "inferred from system reconstruction" RELATED []
synonym: "ISR" RELATED []
is_a: ECO:0000000 ! evidence
created_by: mgiglio
creation_date: 2009-03-20T12:00:17Z
[Term]
id: ECO:0000089
name: restriction landmark genomic scanning evidence
synonym: "RLGS evidence" EXACT []
is_a: ECO:0000073 ! experimental genomic evidence
[Term]
id: ECO:0000090
name: immunogold labelling evidence
def: "Used when an annotation is made based on immunolocalization method that detects an antigen in cytological preparations by using a gold-labeled secondary antibody." [TAIR:TED]
synonym: "IDA: immunogold labelling" RELATED []
is_a: ECO:0000040 ! immunological assay evidence
[Term]
id: ECO:0000092
name: epitope-tagged protein immunolocalization evidence
def: "Used when an annotation is made based on immunolocalization of recombinant proteins fused with epitopes that are recognized by antibodies." [TAIR:TED]
synonym: "IDA: immunolocalization of epitope-tagged protein" RELATED []
is_a: ECO:0000087 ! immunolocalization evidence
[Term]
id: ECO:0000093
name: oligonucleotide microarray evidence
is_a: ECO:0000055 ! array experiment evidence
[Term]
id: ECO:0000094
name: biological assay evidence
def: "Direct assay evidence that is derived by using a living organism to measure the effect of a substance, factor, or condition." [ECO:MCC, TAIR:TED]
synonym: "IDA: bioassay" RELATED []
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000096
name: electrophoretic mobility shift assay evidence
def: "Used when an annotation is made based on electrophoretic mobility shift of macromolecules, compounds, and proteins. Most commonly used for detecting the binding of protein to DNA." [TAIR:TED]
synonym: "IDA: electrophoretic mobility shift assay" RELATED []
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000097
name: cDNA microarray evidence
is_a: ECO:0000055 ! array experiment evidence
[Term]
id: ECO:0000098
name: in situ hybridization evidence
def: "Used when an annotation is made based on a method that hybridizes a labeled nucleic acid probe to a tissue or cell specimen in which the cellular structures are still intact. Depending on the label and protocol used, the probe may detect complementary DNA or RNA by hybridizing specifically to that gene or sequence." [TAIR:TED]
synonym: "IDA: in situ hybridization" RELATED []
is_a: ECO:0000026 ! nucleic acid hybridization evidence
[Term]
id: ECO:0000100
name: fractionation evidence
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000101
name: Affymetrix array experiment evidence
is_a: ECO:0000055 ! array experiment evidence
[Term]
id: ECO:0000102
name: co-fractionation evidence
def: "Used when an annotation is made based on fractionation of a protein with other compounds, factors, or macromolecules." [TAIR:TED]
synonym: "IDA: co-fractionation" RELATED []
is_a: ECO:0000100 ! fractionation evidence
[Term]
id: ECO:0000104
name: microarray RNA expression level evidence
def: "Used when an annotation is made based on levels of RNA expression determined by microarray experiment." [TAIR:TED]
synonym: "IEP: transcript levels (e.g. microarray data)" RELATED []
is_a: ECO:0000048 ! transcript expression level evidence
is_a: ECO:0000058 ! expression microarray evidence
[Term]
id: ECO:0000105
name: Nimblegen array evidence
is_a: ECO:0000055 ! array experiment evidence
[Term]
id: ECO:0000106
name: Northern assay evidence
def: "Used when an annotation is made based on levels of RNA expression determined by a Northern blot experiment." [TAIR:TED]
synonym: "IEP: transcript levels (e.g. Northerns)" RELATED []
is_a: ECO:0000048 ! transcript expression level evidence
[Term]
id: ECO:0000108
name: reverse transcription polymerase chain reaction transcription evidence
def: "Used when an annotation is made based on levels of RNA expression determined by an RT-PCR experiment (quantitative or otherwise)." [TAIR:TED]
synonym: "IEP: transcript levels (e.g. RT-PCR)" RELATED []
synonym: "RT-PCR transcription evidence" EXACT []
is_a: ECO:0000048 ! transcript expression level evidence
is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence
[Term]
id: ECO:0000109
name: reverse transcription polymerase chain reaction evidence
synonym: "RT-PCR evidence" EXACT []
is_a: ECO:0000082 ! polymerase chain reaction evidence
[Term]
id: ECO:0000110
name: RNA protection assay evidence
def: "Used when an annotation is made based on levels of RNA expression determined by an RNA protection assay." [TAIR:TED]
synonym: "IEP: RNA protection assay" RELATED []
is_a: ECO:0000009 ! transcript expression evidence
[Term]
id: ECO:0000112
name: Western blot evidence
def: "Used when an annotation is made based on levels of protein expression determined by a Western blot assay." [TAIR:TED]
synonym: "IEP: protein levels (e.g. Western blots)" RELATED []
is_a: ECO:0000046 ! protein expression level evidence
[Term]
id: ECO:0000114
name: expression library screen evidence
def: "Used when an annotation is made on the basis of a protein expression library screen where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein." [TAIR:TED]
synonym: "IEP: expression library screening" RELATED []
is_a: ECO:0000010 ! protein expression evidence
is_a: ECO:0000040 ! immunological assay evidence
[Term]
id: ECO:0000116
name: differential hybridization evidence
def: "Used when an annotation is made based on results of a differential hybridization experiment that demonstrates expression of an RNA under one condition and not another." [TAIR:TED]
synonym: "IEP: differential hybridization" RELATED []
is_a: ECO:0000009 ! transcript expression evidence
is_a: ECO:0000026 ! nucleic acid hybridization evidence
[Term]
id: ECO:0000118
name: subtractive hybridization evidence
def: "Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another." [TAIR:TED]
synonym: "IEP: subtractive hybridization" RELATED []
is_a: ECO:0000009 ! transcript expression evidence
is_a: ECO:0000026 ! nucleic acid hybridization evidence
[Term]
id: ECO:0000120
name: over expression analysis evidence
def: "Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress the gene product in question." [TAIR:TED]
synonym: "IMP: analysis of overexpression/ectopic expression phenotype" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000122
name: protein localization evidence
is_a: ECO:0000039 ! protein assay evidence
[Term]
id: ECO:0000124
name: fusion protein localization evidence
is_a: ECO:0000122 ! protein localization evidence
[Term]
id: ECO:0000126
name: green fluorescent protein fusion protein localization evidence
synonym: "GFP fusion protein localization evidence" EXACT []
synonym: "IDA: localization of GFP/YFP fusion protein" RELATED []
is_a: ECO:0000124 ! fusion protein localization evidence
[Term]
id: ECO:0000128
name: yellow fluorescent protein fusion protein localization evidence
synonym: "IDA: localization of GFP/YFP fusion protein" RELATED []
synonym: "YFP fusion protein localization evidence" EXACT []
is_a: ECO:0000124 ! fusion protein localization evidence
[Term]
id: ECO:0000130
name: beta-glucuronidase fusion protein localization evidence
synonym: "GUS fusion protein localization evidence" EXACT []
synonym: "GUS staining evidence" NARROW []
synonym: "IDA: localization of GUS fusion protein" RELATED []
is_a: ECO:0000124 ! fusion protein localization evidence
[Term]
id: ECO:0000132
name: beta-galactosidase fusion protein localization evidence
synonym: "LacZ fusion protein localization evidence" EXACT []
is_a: ECO:0000124 ! fusion protein localization evidence
[Term]
id: ECO:0000134
name: transport assay evidence
synonym: "IDA: transport assay" RELATED []
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000136
name: nucleic acid binding evidence
is_a: ECO:0000023 ! ligand binding evidence
[Term]
id: ECO:0000138
name: ribohomopolymer binding assay evidence
synonym: "IDA: ribohomopolymer binding assay" RELATED []
is_a: ECO:0000136 ! nucleic acid binding evidence
[Term]
id: ECO:0000140
name: thin layer chromatography evidence
synonym: "IDA: thin layer chromatography" RELATED []
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000142
name: protein:ion binding evidence
is_a: ECO:0000024 ! protein binding evidence
[Term]
id: ECO:0000144
name: Southwestern blot evidence
def: "Used when an annotation is made based on a Southwestern blot analysis, which detects DNA binding of proteins using labeled DNA as probe hybridizing to electrophoretically separated proteins." [TAIR:TED]
synonym: "IDA: Southwestern analysis" RELATED []
is_a: ECO:0000136 ! nucleic acid binding evidence
[Term]
id: ECO:0000146
name: Northwestern blot evidence
def: "Used when an annotation is made based on a Northwestern blot analysis, which detects RNA binding of proteins using labeled RNA as probe hybridizing to electrophoretically separated proteins." [TAIR:TED]
synonym: "IDA: Northwestern analysis" RELATED []
is_a: ECO:0000136 ! nucleic acid binding evidence
[Term]
id: ECO:0000148
name: in vitro binding evidence
synonym: "IDA: in vitro binding assay" RELATED []
is_a: ECO:0000021 ! physical interaction evidence
[Term]
id: ECO:0000150
name: in vitro transcription reconstitution assay evidence
synonym: "IDA: in vitro reconstitution assay with recombinant protein" RELATED []
is_a: ECO:0000003 ! reconstitution assay evidence
[Term]
id: ECO:0000152
name: in vitro recombinant protein transcription reconstitution assay evidence
synonym: "IDA: in vitro reconstitution assay with recombinant protein" RELATED []
is_a: ECO:0000150 ! in vitro transcription reconstitution assay evidence
[Term]
id: ECO:0000154
name: heterologous protein expression evidence
synonym: "IDA: protein expression in heterologous system" RELATED []
is_a: ECO:0000010 ! protein expression evidence
[Term]
id: ECO:0000156
name: protein separation evidence
is_a: ECO:0000100 ! fractionation evidence
[Term]
id: ECO:0000158
name: protein separation followed by direct sequencing evidence
synonym: "IDA: protein separation and direct sequencing" RELATED []
is_a: ECO:0000156 ! protein separation evidence
[Term]
id: ECO:0000160
name: protein separation followed by fragment identification evidence
synonym: "IDA: protein separation and fragment identification" RELATED []
is_a: ECO:0000156 ! protein separation evidence
[Term]
id: ECO:0000162
name: heterologous system uptake evidence
def: "Used when an annotation is made based on small molecule update assays carried out in a heterologous system that contains a recombinant protein." [TAIR:TED]
synonym: "IDA: uptake assay in heterologous system" RELATED []
is_a: ECO:0000134 ! transport assay evidence
[Term]
id: ECO:0000164
name: electrophysiology assay evidence
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000166
name: two-electrode voltage clamp experiment evidence
synonym: "IDA: two-electrode voltage clamp technique" RELATED []
is_a: ECO:0000164 ! electrophysiology assay evidence
[Term]
id: ECO:0000168
name: transcription assay
is_a: ECO:0000002 ! direct assay evidence
[Term]
id: ECO:0000170
name: transcriptional activation assay
def: "Used when an annotation is made based on assays that show transcriptional activation of a specific target reporter gene." [TAIR:TED]
synonym: "IDA: transcriptional activation assay" RELATED []
is_a: ECO:0000168 ! transcription assay
[Term]
id: ECO:0000172
name: biochemical trait analysis evidence
def: "Used when an annotation is made based on the analysis of a biochemical aspect of a mutant phenotype; for example, the accumulation of a biosynthetic intermediate." [TAIR:TED]
synonym: "IMP: analysis of biochemical trait" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000174
name: mutant physiological response evidence
def: "Used when an annotation is made based on the physiological response of a mutant to an external stimulus; for example, abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions." [TAIR:TED]
synonym: "IMP: analysis of physiological response" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000176
name: mutant visible phenotype evidence
def: "Used when an annotation is made based on the visual examination of a mutant phenotype; for example, abnormalities in organism morphology." [TAIR:TED]
synonym: "IMP: analysis of visible trait" RELATED []
is_a: ECO:0000059 ! experimental phenotypic evidence
[Term]
id: ECO:0000177
name: genomic context evidence
def: "An evidence type that is based on the location of a gene, which gives rise to a gene product, within the genome." [ECO:go, ECO:MCC]
comment: This type of evidence might include identity of neighboring genes, operon structure, synteny, phylogenetic analysis, or other whole-genome analysis.
is_a: ECO:0000000 ! evidence
[Term]
id: ECO:0000178
name: in vivo assay evidence
is_a: ECO:0000094 ! biological assay evidence
[Term]
id: ECO:0000179
name: animal model system study evidence
def: "A type of experimental evidence arising from the investigation of an animal model system." [ECO:MCC]
is_a: ECO:0000006 ! experimental evidence
[Term]
id: ECO:0000180
name: clinical study evidence
def: "A type of experimental evidence arising from a controlled investigation that uses human subjects." [ECO:MCC]
is_a: ECO:0000006 ! experimental evidence
[Term]
id: ECO:0000181
name: in vitro assay evidence
is_a: ECO:0000094 ! biological assay evidence
[Term]
id: ECO:0000182
name: in vitro culture assay evidence
synonym: "ex vivo assay" RELATED []
is_a: ECO:0000094 ! biological assay evidence
[Term]
id: ECO:0000183
name: cell-free assay evidence
synonym: "in vitro assay" BROAD []
is_a: ECO:0000181 ! in vitro assay evidence
[Term]
id: ECO:0000184
name: enzyme inhibition experiment evidence
is_a: ECO:0000005 ! enzyme assay evidence
is_a: ECO:0000020 ! specific protein inhibition experiment evidence
[Term]
id: ECO:0000200
name: sequence alignment evidence
alt_id: ECO:00000057
def: "Used when a sequence alignment is the basis for making an annotation, but only when the alignment has been manually reviewed; or if the information is in a published paper, the authors have manually reviewed the evidence. Such alignments may be pairwise alignments or multiple alignments. BLAST produces pairwise alignments and any annotations based solely on the evaluation of BLAST results should use this code." [url:http\://www.geneontology.org/GO.evidence.shtml]
synonym: "inferred from sequence alignment" EXACT [GO:ISA]
synonym: "ISA" EXACT [GO:ISA]
is_a: ECO:0000044 ! sequence similarity evidence
created_by: mchibucos
creation_date: 2010-03-18T12:21:31Z
[Term]
id: ECO:0000201
name: sequence orthology evidence
alt_id: ECO:00000060
def: "Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. Orthology is established by multiple criteria generally including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors." [url:http\://www.geneontology.org/GO.evidence.shtml]
synonym: "inferred from sequence orthology" EXACT [GO:ISO]
synonym: "ISO" EXACT [GO:ISO]
synonym: "ortholog evidence" RELATED []
is_a: ECO:0000044 ! sequence similarity evidence
created_by: mchibucos
creation_date: 2010-03-18T12:30:06Z
[Term]
id: ECO:0000202
name: match to sequence model evidence
alt_id: ECO:00000063
def: "Sequence similarity evidence that is based on a sequence-based statistical model that predicts the function of a gene product." [ECO:MCC, url:http\://www.geneontology.org/GO.evidence.shtml]
comment: Used when evidence from any kind of statistical model of a sequence or group of sequences is used to make a prediction about the function of a protein or RNA. Examples of relevant evidence are: Hidden Markov Models, PROSITE motifs, and tools such as tRNASCAN.
synonym: "inferred from sequence model" EXACT [GO:ISM]
synonym: "ISM" EXACT [GO:ISM]
is_a: ECO:0000044 ! sequence similarity evidence
created_by: mchibucos
creation_date: 2010-03-18T12:32:30Z