diff --git a/MakeExecutables.bat b/MakeExecutables.bat index 65f829c..db32d1d 100755 --- a/MakeExecutables.bat +++ b/MakeExecutables.bat @@ -20,12 +20,8 @@ :: See the file README.MD for how to set up your anaconda environment. -:: Please verify that your files are set up such that the files exist here:: -:: C:\MinIONScripts\AlleleSubmission\MakeExecutables.bat -:: If that is a problem, Modify the spec file "AlleleSubInstallerOptions_Windows.spec" as your needs require. - -SET CodePath=C:\MUMCScripts\EMBL-HLA-Submission\src -SET BinPath=C:\MUMCScripts\EMBL-HLA-Submission\bin +SET CodePath=src +SET BinPath=..\bin SET SpecFile=AlleleSubInstallerOptions_Windows.spec SET CondaEnvironment=AlleleSubEnvironment diff --git a/README.md b/README.md index 1be2884..70b453d 100644 --- a/README.md +++ b/README.md @@ -41,12 +41,12 @@ Run_allele_submission.bat ``` ##Input Data -Input data sequence consists of +Input data sequence must consist of 1) 5' UTR -2) An odd number of Exons, with an Even number of introns between them. +2) Any number of Exons, with an introns distributed between them. 3) 3' UTR -Introns, Exons, and UTRs are to be specified using capital or lowercase letters. +Introns, Exons, and UTRs are to be distinguished using capital or lowercase nuclotide letters. Exons are capital, while introns and UTRs are lowercase. Like this: @@ -54,7 +54,8 @@ fiveprimeutrEXONONEintrononeEXONTWOintrontwoEXONTHREEthreeprimeutr agctagctagctAGCTAGCtagctagctAGCTAGCtagctagctAGCTAGCTAgctagctagctag All spaces, tabs, and newlines are not interpreted by this program, They are removed. -If you wish, you may use those characters for more convenient visualization of splice sites: +If you wish, you may use those characters for convenient visualization of splice sites, like this: + agctagctagct AGCTAGC tagctagct @@ -64,7 +65,7 @@ AGCTAGCTA gctagctagctag ##Output Data -The resulting report is in the form of an EMBL HLA Novel Allele submission flatfile. You can submit this to EMBL as a new allele. +The resulting report is in the form of an EMBL HLA Novel Allele submission flatfile. You can submit this to EMBL as a new HLA allele. ##For more information on EMBL's ENA format: http://www.ebi.ac.uk/ena/submit/sequence-submission diff --git a/Run_allele_submission.bat b/Run_allele_submission.bat index 24be771..a7152a8 100755 --- a/Run_allele_submission.bat +++ b/Run_allele_submission.bat @@ -17,5 +17,5 @@ :: See the file README.MD for how to set up your anaconda environment. -activate AlleleSubEnvironment && python AlleleSubmissionEMBL.py && deactivate +activate AlleleSubEnvironment && cd src && python AlleleSubmissionEMBL.py && deactivate