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Question about the [Introduction/Raw data processing 3.8] #307

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Wenxi-Zhang opened this issue Nov 20, 2024 · 0 comments
Open

Question about the [Introduction/Raw data processing 3.8] #307

Wenxi-Zhang opened this issue Nov 20, 2024 · 0 comments
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question Further information is requested

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@Wenxi-Zhang
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Wenxi-Zhang commented Nov 20, 2024

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Hello! I try to replicate the real world example listed in 3.8 but faced the issue when using simpleaf at step 3.8.2. I used macOS sonoma 14.7, M2 chip.
So I tried simpleaf set-paths and get this:
found piscem in the PATH at /opt/anaconda3/envs/af/bin/piscem
found salmon in the PATH at /opt/anaconda3/envs/af/bin/salmon
found alevin-fry in the PATH at /opt/anaconda3/envs/af/bin/alevin-fry
Then I tried simpleaf index \ -o simpleaf_index \ -f toy_human_ref/fasta/genome.fa \ -g toy_human_ref/genes/genes.gtf \ -r 90 \ -t 8
and get this:
Image
Image

Could you help me with this? Thank you!

@Wenxi-Zhang Wenxi-Zhang added the question Further information is requested label Nov 20, 2024
@Wenxi-Zhang Wenxi-Zhang reopened this Nov 20, 2024
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