Adding SILAC label in quantitation configuration

[BrandonMurugan]

Hi there,

I was wondering how I would go about adding SILAC modifications to the quantitation configuration?
I'm having some trouble figuring out the 15N configuration and adapting it for SILAC.

Thanks for the great piece of software!

Brandon

[01joy]

Dear @BrandonMurugan ,

If your SILAC labeling is ¹³C₆¹⁵N₄ arginine (Arg10) and ¹³C₆¹⁵N₂ lysine (Lys8), please add the following configuration in quantitation of pConfig.

Name: SILAC-Arg10Lys8
Label info: R:K{N,15N}R:K{C,13C}R:R{N,15N}R:R{C,13C}

For the rules of label info, every R:K{N,15N} is a rule, it means: for residue (R) Lys (K), replace its N as 15N. You can see in Amino Acids tab of pConfig that K contains two Ns, so this rule will replace two Ns as two 15Ns.