EF_yl_tXPR2-part.gb

LOCUS       EF_yl_tXPR2                 310 bp    DNA     linear   UNC 08-SEP-2022
DEFINITION  Yarrowia lipolytica XPR2 native terminator as an EF (GCTT/CGCT) part.
ACCESSION   .
VERSION     .
KEYWORDS    Open Yeast Collection IIA Part; Golden Gate Assembly;
            OpenMTA; iGEM;
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Scott Pownall, Open Science Network Society
  TITLE     Direct Submission 
  JOURNAL   https://iGEM.org/  
REFERENCE   2  (bases 44 to 246)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T.
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. 
            doi: 10.1111/1751-7915.13427.
            PubMed ID:  31148366
COMMENT     Part for clonal synthesis; Random sequence added to meet Twist's minimum.
            BbsI sites for cloning in pOpen_v3; PacI/SpeI for SEVA-based vectors.
FEATURES             Location/Qualifiers
     protein_bind    join(4..9,12..15)
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    17..24
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    26..31
                     /label="XbaI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    33..38
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    40..43
                     /label="E"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      40..250
                     /label="XPR2 terminator"
                     /original_record="part"
     misc_feature    247..250
                     /label="F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(252..257)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    258..263
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    complement(join(266..269,272..277))
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    278..310
                     /label="random sequence"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ggcGAAGACa gGGAGcTTAA TTAAcTCTAG AcGGTCTCtG CTTatcggcc cgggcaaacc
       61 acaccaaccc ttctcttcta tcgccagtcg aaatgtgact ttttaagtaa tcaattcttg
      121 taaacacttg atcgtacgta gttggatcat ggcgcgacaa aataatttcc aaccagtgtc
      181 actcactcca ctcagactgt tggagcactt gacagataga tcacccatca agcagcaact
      241 tggaatCGCT tGAGACCact agtcgCGCTt cGTCTTCtga ctatagtgta acgcaggttc
      301 gtctgcccta
//