EF_yl_tGuo-part.gb

LOCUS       EF_yl_tGuo                  310 bp    DNA     linear   UNC 12-SEP-2022
DEFINITION  Yarrowia lipolytica Guo short synthetic terminator as a EF (GCTT/CGCT) part.
ACCESSION   .
VERSION     .
KEYWORDS    Open Yeast Collection IIA Part; Golden Gate Assembly;
            OpenMTA; iGEM;
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Scott Pownall, Open Science Network Society
  TITLE     Direct Submission 
  JOURNAL   https://iGEM.org/  
REFERENCE   2  (bases 44 to 82)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T.
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. 
            doi: 10.1111/1751-7915.13427.
            PubMed ID:  31148366
COMMENT     Part for clonal synthesis; Random sequence added to meet Twist's minimum.
            BbsI sites for cloning in pOpen_v3; PacI/SpeI for SEVA-based vectors.
FEATURES             Location/Qualifiers
     protein_bind    join(4..9,12..15)
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    17..24
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    33..38
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    40..43
                     /label="E"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      40..86
                     /label="Guo terminator"
                     /original_record="part"
     misc_feature    83..86
                     /label="F"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(88..93)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    94..99
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    complement(join(102..105,108..113))
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    114..310
                     /label="random sequence"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ggcGAAGACa gGGAGcTTAA TTAAcTCTAG AcGGTCTCtG CTTtatataa ctgtctagaa
       61 ataaagagta tcatctttca aaCGCTtGAG ACCactagtc gCGCTtcGTC TTCtgatgat
      121 ctaatgttct agggaccaca cctcgaagag gtgcgaaccg gattgttagt atgcatatga
      181 acgtGaCCtG tgacttgatg tcagcattgc cgcttcacta gagaagcttt gcagactcgc
      241 tgaaactcca cttgtttgca tctataaccc agctaacgct gtttaagcac cgaggcccat
      301 ttaggaagtg 
//