BT1_alpha_amylase_signal.gb

LOCUS       BT1_alpha_amylase_signal 310 bp    DNA     linear   UNK 12-MAR-2023
DEFINITION  α-amylase signal sequence from Aspergillus niger fused to
            αMF_no_EAEA. BT1 syntax TACT/CCAT.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit; Golden Gate Assembly; OpenMTA; iGEM; 
            Open Yeast Collection IIA Part.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Scott Pownall, Ian Caven, Open Science Network Society
  TITLE     Direct Submission
  JOURNAL   https://iGEM.org/
REFERENCE   2  (bases 44 to 274)
  AUTHORS   Ulrike Obst, Timothy K Lu, Volker Sieber
  TITLE     A Modular Toolkit for Generating Pichia pastoris Secretion Libraries.
  JOURNAL   ACS Synth Biol. 2017 Jun 16;6(6):1016-1025
            PubMed ID: 28252957
			doi: 10.1021/acssynbio.6b00337
COMMENT     .
FEATURES             Location/Qualifiers
     protein_bind    join(4..9,12..15)
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    17..24
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    26..31
                     /label="XbaI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    33..38
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    40..43
                     /label="B"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             44..103
                     /codon_start=1
                     /label="α-amylase signal"
                     /translation="MVAWWSLFLYGLQVAAPALA"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     CDS             104..274
                     /codon_start=1
                     /label="αMF_no_EAEA"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDELEGDFDVAVLPFSASI
                     AAKEEGVSLEKR"
                     /ApEinfo_fwdcolor="#2B8AE2"
                     /ApEinfo_revcolor="#2B8AE2"
     misc_feature    276..279
                     /label="T1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    complement(281..286)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    287..292
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    complement(join(295..298,301..306))
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 ggcGAAGACa gGGAGcTTAA TTAAcTCTAG AcGGTCTCcT ACTatggtgg catggtggtc
       61 cttattctta tatggacttc aagttgctgc tcctgccctt gctatgagat ttcctagtat
      121 tttcactgct gtgctatttg ccgctagttc cgctctagct gctccagtta atactactac
      181 tgaagatgaa ttggagggtg acttcgatgt tgctgttctg cctttttccg cttctatcgc
      241 agccaaggaa gaaggtgtat ccctagagaa gcgttCCATg GAGACCacta gtcgCGCTtc
      301 GTCTTCtgat
//