BT1_Wci_α_mating_factor_signal.gb

LOCUS       BT1_Wci_α_mating_factor_signal 363 bp    DNA     linear   UNK 12-MAR-2023
DEFINITION  α-mating factor secretion signal from Wickerhamomyces ciferrii. BT1
            syntax TACT/CCAT.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit; Golden Gate Assembly; OpenMTA; iGEM; 
            Open Yeast Collection IIA Part.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Scott Pownall, Ian Caven, Open Science Network Society
  TITLE     Direct Submission
  JOURNAL   https://iGEM.org/
REFERENCE   2  (bases 44 to 328)
  AUTHORS   Chenwei Zou, Lingfang Lu, Shengyan Wang, Chenshan Zhang, Xuequn Chen, 
            Yao Lin, Yide Huang.
  TITLE     The α-mating factor secretion signals and endogenous signal peptides 
            for recombinant protein secretion in Komagataella phaffii.
  JOURNAL   Biotechnol Biofuels Bioprod. 2022 Dec 16;15(1):140
            PubMed ID: 36527112
			doi: 10.1186/s13068-022-02243-6
FEATURES             Location/Qualifiers
     protein_bind    join(4..9,12..15)
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    17..24
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    26..31
                     /label="XbaI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    33..38
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    40..43
                     /label="B"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             44..106
                     /codon_start=1
                     /label="W.ci. αMF signal"
                     /translation="MQLSLLTSLAIVSTLLGSSFA"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     CDS             107..328
                     /codon_start=1
                     /label="W.ci. αMF pro peptide"
                     /translation="APVENINIKDNGNGTSEADVPGTSQGVEFPFAKEAIIEAVSLGND
                     IAPIVLNDAVYFVNTTTVDKELESKLGKR"
                     /ApEinfo_fwdcolor="#2B8AE2"
                     /ApEinfo_revcolor="#2B8AE2"
     misc_feature    330..333
                     /label="T1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    complement(335..340)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    341..346
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    complement(join(349..352,355..360))
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 ggcGAAGACa gGGAGcTTAA TTAAcTCTAG AcGGTCTCcT ACTatgcaac tttctttact
       61 aacttctttg gccattgttt ccactttact aggctcttcg tttgctgctc cagtggagaa
      121 tatcaatatc aaagacaatg gcaatggcac cagtgaagct gatgttcctg gaacttctca
      181 aggtgttgag tttccgtttg ccaaagaagc catcattgaa gctgtttctt taggcaatga
      241 tattgctcca attgttctca atgatgctgt ttattttgtc aataccacca ctgttgacaa
      301 agagctggaa agcaaacttg gcaaacgatC CATgGAGACC actagtcgCG CTtcGTCTTC
      361 tga
//