BC_His_TEVcut.gb

LOCUS       BC_His_TEVcut            310 bp    DNA     linear   SYN 03-NOV-2022
DEFINITION  A BCD combined with a his N terminal purification tag and a TEV
            cleavage site. Defined as a BC (TACT/AATG) part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit; Golden Gate Assembly; OpenMTA; iGEM; 
            Open Yeast Collection IIA Part.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Scott Pownall, Ian Caven, Open Science Network Society
  TITLE     Direct Submission
  JOURNAL   https://iGEM.org/
REFERENCE   2  (bases 44 to 108)
  AUTHORS   Dr. Jenny Molloy and collaborators, openbioeconomylab.org
  TITLE     E.coli Protein Expression Toolkit.
  JOURNAL   https://freegenes.github.io/genes/BBF10K_003419.html
COMMENT     Adapted for yeast from the E.coli Protein Expression Toolkit sans RBS.
FEATURES             Location/Qualifiers
     protein_bind    join(4..9,12..15)
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    17..24
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    26..31
                     /label="XbaI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    33..38
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    40..43
                     /label="B"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             44..108
                     /label="BC_His_TEVcut"
                     /gene="BC_His_TEVcut"
                     /translation="MGSSHHHHHHSSGGENLYFQGGM"
     misc_feature    109..112
                     /label="C"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    complement(114..119)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    120..125
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    complement(join(128..131,134..139))
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    143..310
                     /label="random sequence"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
ORIGIN
        1 ggcGAAGACa gGGAGcTTAA TTAAcTCTAG AcGGTCTCcT ACTatgggat cttctcatca
       61 ccatcatcat cattcctctg gaggagagaa cttgtatttt cagggtggAA TGaGAGACCa
      121 ctagtcaCGC TtcGTCTTCt gagagaggcc ctacaaggaa atagcgtact ttagttcgga
      181 cgcccagtgt tcacaaggac accgccccag atcgtactat catgtattcc tgcgatggcc
      241 tcaataattg ccttaccagt gaactgtcac tgaatcacct accgctttcg tgctttactg
      301 taaacaggga
//