AB_yl_pGAPDH-part.gb

LOCUS       AB_yh_pGAPDH                880 bp    DNA     linear   UNC 05-SEP-2022
DEFINITION  Yarrowia lipolytica GAPDH promoter as an AB (GGAG/TACT) part.
ACCESSION   .
VERSION     .
KEYWORDS    Yarrowia lipolytica, Golden Gate Assembly; OpenMTA; iGEM; 
            Open Yeast Collection IIA Part; 
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Scott Pownall, Ian Caven, Open Science Network Society
  TITLE     Direct Submission
  JOURNAL   https://iGEM.org/
REFERENCE   2  (bases 44 to 850)
  AUTHORS   Lynn Wong, Jake Engel, Erqing Jin, Benjamin Holdridge, Peng Xu
  TITLE     YaliBricks, a versatile genetic toolkit for streamlined and rapid pathway 
            engineering in Yarrowia lipolytica.
  JOURNAL   Metab Eng Commun. 2017 Oct 1;5:68-77
            PubMed ID: 29188186
			doi: 10.1016/j.meteno.2017.09.001
COMMENT     BsmBI site removed (t>A); BbsI site removed (t>A); SpeI site removed (t>A)
FEATURES             Location/Qualifiers
     protein_bind    join(4..9,12..15)
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    17..24
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    26..31
                     /label="XbaI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    33..38
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    40..43
                     /label="A"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        40..850
                     /label="GAPDH Promoter"
                     /original_record="part"
     misc_feature    847..850
                     /label="B"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(852..857)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    858..863
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    complement(join(866..869,872..877))
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 ggcGAAGACa gGGAGcTTAA TTAAcTCTAG AcGGTCTCtG GAGtaccaac cacagattac
       61 gacccattcg cagtcacagt tcactagggt ttgggttgca tccgttgaga gtggtttgtt
      121 tttaaccttc tccatgtgct cactcaggtt ttgggttcag atcaaatcaa ggcgtgaacc
      181 actgtttgag gacaaatgtg acacaaccaa ccagtgtcag gggcaagtcc gtgacaaagg
      241 ggaagataca atgcaattac tgacagttac ggactgcctc gatgccctaa ccttgcccca
      301 aaataagaca actgtcctcg tttaagcgca accctattca gcgtcacgtc ataatagcgt
      361 ttggatagca ctagActatg aggagcgttt tatgttgcgg tgagggcgat tggtgctcat
      421 atgggAtcaa ttgaggtggt ggaacgagct tagtctAcaa ttgaggtgcg agcgacacaa
      481 ttgggtgtca cgtggcctaa ttgacctcgg atcgtggagt ccccagttat acagcaacca
      541 cgaggtgcat gagtaggTga cgtcaccaga caatagggtt tttttggact ggagagggta
      601 gggcaaaagc gctcaacggg ctgtttgggg agctatgggg gaggaattgg cgatatttgt
      661 gaggttgacg gctccgattt gcgtgttttg tcgcttctgc atctccccat acccatatct
      721 tccctcccca cctctttcca cgataatttt acggatcagc aataaggttc cttctcctag
      781 tttccacgtc catatatatc tatgctgcgt cgtccttttc gtgacatcac caaaacacat
      841 acaaaaTACT tGAGACCact agtcgCGCTt cGTCTTCtga
//