AA2_yl_UAS1B-part.gb

LOCUS       AA2_yl_UAS1B-part               310 bp    DNA     linear   UNC 11-FEB-2023
DEFINITION  Yarrowia lipolytica upstream activating sequence UAS1B as an AA2   
            (GGAG/TAAC) part. To be used with A2B minimal promoters.
ACCESSION   .
VERSION     .
KEYWORDS    Open Yeast Collection IIA Part; Golden Gate Assembly; OpenMTA; iGEM;
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Scott Pownall, Open Science Network Society
  TITLE     Direct Submission 
  JOURNAL   https://iGEM.org/
REFERENCE   2  (bases 44 to 188)
  AUTHORS   Murtaza Shabbir Hussain, Lauren Gambill, Spencer Smith, and Mark Blenner
  TITLE     Engineering Promoter Architecture in Oleaginous Yeast Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Mar 18;5(3):213-23.
            doi: 10.1021/acssynbio.5b00100
            PubMed ID: 26635071
REFERENCE   3  (bases 44 to 188)
  AUTHORS   John Blazeck, Ben Reed, Rishi Garg, Rachelle Gerstner, Annie Pan, 
            Vaibhav Agarwala, Hal S. Alper
  TITLE     Generalizing a hybrid synthetic promoter approach in Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2016 Mar 18;5(3):213-23.
            doi: 10.1007/s00253-012-4421-5
            PubMed ID: 23053080
COMMENT     Part for clonal synthesis; Random sequence added to meet Twist's minimum. 
            BsmBI dropout is used for recursive assembly of multiple UAS units. 
            Internal BsaI site removed (a>T).
FEATURES             Location/Qualifiers
     protein_bind    join(4..9,12..15)
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    17..24
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    26..31
                     /label="XbaI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    33..38
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    40..43
                     /label="A"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(45..50)
                     /label="BsmBI"
                     /ApEinfo_fwdcolor="#8B008B"
                     /ApEinfo_revcolor="#8B008B"
     misc_feature    51..68
                     /label="BsmB1 dropout"
                     /ApEinfo_fwdcolor="#A9A9A9"
                     /ApEinfo_revcolor="#A9A9A9"
     protein_bind    69..74
                     /label="BsmBI"
                     /ApEinfo_fwdcolor="#8B008B"
                     /ApEinfo_revcolor="#8B008B"
     misc_feature    76..79
                     /label="A2"
                     /ApEinfo_revcolor=#A9A9A9
                     /ApEinfo_fwdcolor=#A9A9A9
     promoter        80..184
                     /label="UAS1B"
                     /original_record="part"
     misc_feature    185..188
                     /label="A2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    complement(190..195)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    196..201
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    complement(join(204..207,210..215))
                     /label="BbsI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    216..310
                     /label="random sequence"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 ggcGAAGACa gGGAGcTTAA TTAAcTCTAG AcGGTCTCtG GAGtGAGACG ctcgagGTGC
       61 ACcaattgCG TCTCgTAACc tgaggtgtct cacaagtgcc gtgcagtccc gcccccactt
      121 gcttctcttt gtgtgtagtg tacgtacatt atcgTgaccg ttgttcccgc ccacctcgat
      181 ccggTAACtG AGACCactag tcgCGCTtcG TCTTCtgact atagtgtaac gcaggttcgt
      241 ctgccctagt ctctgacctc gacgggcacg atgatacgga ctgctaggta gaggctagtt
      301 acgccctgcc
//