T3C_yTEVcut.gb

LOCUS       T3C_yTEVcut              300 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  A N terminal TEV cleavage site. Defined as a T3C part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM.
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society. Based on a part in the OpenBioeconomy Lab's 
            E. coli Protein Expression Toolkit.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="T3"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             22..42
                     /label="T3C_yTEVcut"
                     /gene="T3C_yTEVcut"
                     /translation="ENLYFQG"
     misc_feature    45..48
                     /label="C"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(50..55)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    56..61
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    62..300
                     /label="random sequence"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
ORIGIN
        1 TTAATTAAGG TCTCcTCCAg cgagaatctg tactttcaag gaggAATGaG AGACCACTAG
       61 Ttggacgtta tctgttgata tgtcgcaacg gctcgccctc ttcccgacac ttcgtaacag
      121 tgcctcttgt gtactgattg gaggcctgga tacggtcggg gtcaccatat cgtgcctatg
      181 ctatcaacat actacccttt tcgcgcagcc gtgaaacgtc agtagatcat ctgcggttac
      241 tgcacatcat gaacctagcc cttaggtctt ggttagagag cggcagttaa tattctaata
//