T3C_yEnterokinase_cut.gb

LOCUS       T3C_yEnterokinase_cut    300 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  Enterokinase is a serine protease (EC 3.4.21.9) that cleaves
            proteins following the lysine at the cleavage site
            Asp-Asp-Asp-Asp-Lys.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM.
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="T3"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             22..39
                     /label="T3C_yEnterokinase_cut"
                     /gene="T3C_yEnterokinase_cut"
                     /translation="GDDDDK"
     misc_feature    42..45
                     /label="C"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(47..52)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    53..58
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    59..300
                     /label="random sequence"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
ORIGIN
        1 TTAATTAAGG TCTCcTCCAg cggtgatgac gatgacaagg gAATGaGAGA CCACTAGTga
       61 gtcggccact gcgcgaagaa tgtagaagga gcgactttgc gcaagccttg ggtctgcact
      121 tgtaccgcca ctgaggcgac attacgtctg cgagagtatc actaattgcg gtgaccaatg
      181 ttaggttctg caaccttgga gactcgttga cgtaaacagc tcgcatctaa ggacgaatcc
      241 tcaggaactt gggctgtgcg attcgaagct cgactggtgc tgacggtaac gtgctggcgg
//