T2C_flex_Kex2_Ste13cut.gb

LOCUS       T2C_flex_Kex2_Ste13cut   300 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  Flexible linker with Kex2 and Ste13 cleavage sites, defined as a T2C
            part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM.
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="T2"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             20..67
                     /label="T2C_flex_Kex2_Ste13cut"
                     /gene="T2C_flex_Kex2_Ste13cut"
                     /translation="GGGGSGGGGSKREAEA"
     misc_feature    70..73
                     /label="C"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(75..80)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    81..86
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     misc_feature    87..300
                     /label="random sequence"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
ORIGIN
        1 TTAATTAAGG TCTCcGTCAg gcggcggcgg tagtggtggt ggtggtagta aacgcgaagc
       61 ggaagcgggA ATGcGAGACC ACTAGTccct atacttatct gtaagacagg tctccaaaag
      121 cgtctagctt cttcagcaag ttctgaaaca aagccactcg cctggatctg catccactgt
      181 acccaggtct aagtctgcat atatctgcaa ggtccctccg atccttgcgt agtgtgataa
      241 tggtgcatta cagcccgctg ctagagccct acccataagt agcaccagct ttagagtctg
//