T1T2_yCipA.gb

LOCUS       T1T2_yCipA               459 bp    DNA     linear   SYN 30-NOV-2023
DEFINITION  CipA cellulose binding domain N-terminal purification tag from the
            E. coli Protein Expression Toolkit, defined as a T1T2 part.
ACCESSION   .
VERSION     .
KEYWORDS    Yeast Protein Expression Toolkit, OpenMTA, Golden Gate Assembly, iGEM.
SOURCE      .
  ORGANISM  .
            .
COMMENT     Codon optimized for Yeast. Sequences were sanitized for PacI, SpeI,
            BsaI, BbsI, BsmBI, and SapI restriction sites. Parts are flanked by
            PacI and SpeI to permit transfer to the SEVA family of vectors.
            Final sequences designed by Ian Cavén and Scott Pownall of Open
            Science Network Society. Based on a part in the OpenBioeconomy Lab's 
            E. coli Protein Expression Toolkit.
FEATURES             Location/Qualifiers
     protein_bind    1..8
                     /label="PacI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
     protein_bind    9..14
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     misc_feature    16..19
                     /label="T1"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     CDS             21..440
                     /label="T1T2_yCipA"
                     /gene="T1T2_yCipA"
                     /translation="NSINPQFKVTNTGSSAIDLSKLTLRYYYTVDGQKDQTFWCDHAAI
                     IGSNGSYNGITSNVKGTFVKMSSSTNNADTYLEISFTGGTLEPGAHVQIQGRFAKNDWS
                     NYTQSNDYSFKSASQFVEWDQVTAYLNGVLVWGKEP"
     misc_feature    443..446
                     /label="T2"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
     protein_bind    complement(448..453)
                     /label="BsaI"
                     /ApEinfo_fwdcolor="#00FF2C"
                     /ApEinfo_revcolor="#00FF2C"
     protein_bind    454..459
                     /label="SpeI"
                     /ApEinfo_fwdcolor="#8A2BE2"
                     /ApEinfo_revcolor="#8A2BE2"
ORIGIN
        1 TTAATTAAGG TCTCcCCATg aactctatta acccccagtt taaggtcact aacactggtt
       61 cttctgctat tgatctgtcc aagttgaccc tgagatacta ctacactgtt gatggtcaga
      121 aggatcaaac cttttggtgt gatcatgctg ccattattgg ttctaacggt tcctacaacg
      181 gtattacctc caacgttaag ggtactttcg ttaagatgtc ctcctctacc aacaacgctg
      241 atacctacct ggaaatttcc tttactggtg gtactctgga accaggtgct catgttcaaa
      301 ttcaaggccg ttttgccaag aacgattggt ctaactacac tcagtccaac gactactctt
      361 ttaagtctgc ttcccagttt gtcgaatggg atcaagttac tgcctacttg aacggtgttt
      421 tggtttgggg taaggaacca ggGTCAaGAG ACCACTAGT
//